Activity of NAD(P)-dependent dedehydrogenases in blood lymphocytes, healthy and tumor lung tissue cells in patients with non-small-cell lung cancer

The aim of the study was to examine enzyme's activity level in blood lymphocytes, healthy and tumor lung tissue cells in patients with lung cancer. NAD and NADP-dependent dehydrogenises activity was studied by bioluminescent method. Dehydrogenases characterizes activity of aerobic and anaerobic reactions and some macromolecular synthesis reactions. We revealed higher dehydrogenases activity in tumor tissue cells, then in healthy tissue. We found similar changes of NAD and NADP-dependent dehydrogenases activity in healthy and tumor lung tissue. Metabolism activity in both tumor and healthy lung tissue did not depend on tumor size. Metabolism of blood lymphocytes in patients with lung cancer was characterized by decreased activity of aerobic and anaerobic energetic processes. Lymphocytes dehydrogenases activity defines the level of bioenergetic and plastic processes in cells. Lymphocytes dehydrogenases activity correlated negatively with tumor size. We proved that regulatory correlation between blood lymphocytes, tumor and healthy lung cells are realized by intercellular metabolism through malat-aspartate shunt and amino acids reactions.