The way of obtaining aseptic explants of potato culture in vitro

The research objective was looking for disinfecting agents ensuring high sterility and safety of primary explant viability. Studying of low-toxic sterilizing substances for receiving primary explant of potatoes in culture in vitro was carried out in the Laboratory of Selection of Krasnoyarsk SAU. For receiving aseptic explant the following agents were used: "Domestos" (5 % sodium hypochlorite, anion, nonionic surfactant, soap, fragrance), "Belizna" (hypochlorite of sodium, active chlorine - 95.2 %), medical antiseptic solution (the volume of ethyl alcohol - 95 %), hydrogen peroxide (36-38 %), "Cefotaxime" - semisynthetic antibiotic of cephalosporin group. Sprouts cultivation on agar nutrient medium was performed according to the prescription of Murashige and Skoog (MS - 1.0, sucrose 20 g/l, casein hydrolyzate) in modification of All-Russia Research and Development Institute of Potato Production. For the establishment of the efficiency of sterilization the explants were examined to ensure the absence of exophyte infection for the 3, 5 and 7-th days after planting...