Comparative analysis of oxygen-dependent and oxygen-independent bactericidal systems of neutrophiles in leukemic infectious disease

The research objective is comparative study of enzymatic (myeloperoxidase) and nonenzymatic (cationic proteins) bactericidal systems activity of neutrophil granulocytes in the cattle with different compromise degree of leukemic infectious disease with the help of cytochemical research methods. The experiment with 25 cows from leukemia contaminated herd has been performed in this regard. 3 groups have been formed under the diagnostic studies of the results with the help of the reaction of immunodiffusion test (RID) and hematological method, i.e. the 1st group includes the animals with negative RID result (control, n=10), the 2nd group includes the animals with positive RID result (n=10) and the 3d group includes sick and leukemia suspected animals under the “leukemic key” (n=5). It is established under the result of the studies performed that decreased neutrophilic granulocytes with high, medium and low degrees of lysosomal cationic proteins (LCP) and myeloperoxidase activity (MPO), and their total number expressed as a middle cytochemical coefficient (MCC) has been observed in bovine leukemia virus (BLV) infected animals. The antimicrobial peptides deficiency increases so far as the leukemic process progress demonstrating more significant coefficient decrease in sick and leukemia suspected animals. The MCC of LCP and MPO decreases by 1.7 and 1.4, respectively, in the BLV carriers as compared with the control group and by 2.1 and 1.8 (p˂0.05) in animals transferred to the hematological stage. Close correlation (r≥0.70) has been found between the enzymatic and nonenzymatic bactericidal systems activity in RID negative and BLV infected cows.