Comparative evaluation of lily varieties by reproduction rate in the culture in vitro

The purpose of the research was to compare the varieties of Asian lilies by the multiplication coefficient in culture in vitro. The objects of the research were 23 varieties of Asian lilies of the selection of the Michurinsk State Research Center, situated in Michurinsk. During experimental work, the methods generally accepted in the research on the culture of the isolated cells, tissues and organs were used. The experiments were laid in 3-fold repetition, 25 plants in each. Micro-plants of the lilies were cultivated on the basis of the mineral part of the Murashige-Skuga nutrient medium with the addition of 6-BAP 1 mg/l. Growing conditions of the plants were maintained at the level of the temperature 20±22 °C, the illumination of 2200 Lux, the duration of the photoperiod was 16 hours. The ability a large number of micro-plants in a short time, in vitro, is largely determined by the coefficient of reproduction of the culture. Each culture has its own coefficient and this indicator is often influenced by genotypic features of the plant, manifested when growing in vitro. As a result of the study, under the same conditions of cultivation of Asian lilies by in vitro method, the varietal specificity was revealed. Thus, there is a significant difference between the varieties in the amount of formation of microtubules. The varieties 'Lyustra' and 'Luchi Nazhezhdy', had a good proliferation, the coefficient of reproduction changed averagely from 7.9 to 7.1. The lowest reproduction rate was 1.3-2.2 and was observed in the varieties 'Anastasia', 'Andromeda', 'Belye Parusa','Volkhova', 'Glafira' and 'Sibiryachka'. The success of rapid reproduction of lilies in vitro in future will depend on the ability of the variety to form a larger number of micro-bulbs.