Cell electroporator

The Institute for Biological Instrumentation has developed a device for cell electroporation that can be used in cellular engineering, biotechnology and biology. The electroporator comprises two electrically connected units: controller and chamber. The controller generates electric signals necessary for the electroporation process. A single pulse or a series of pulses are formed within two ranges. In the first range, the pulse amplitude is 100 to 500 V for the length of 300 μs to 99 ms. In the other one, the amplitude is 500 to 3000 V for the length of 5 μs to 99 μs. Number of pulses in a series varies from 1 to 99. An acoustic warning signal is generated at the end of each pulse train. The chamber is constructed based on a standard Petri dish 90 mm dia. Stainless steel electrodes ensure the best uniformity of electric field. The distance between the electrodes can be varied with a 2-mm step. Total protection against electric shock is provided. The electroporator specimens have been manufactured and tested for compliance with the specifications.