The impact of lead acetate on structural and functional organisation of the anterior and middle intestine of the Siberian sturgeon

Introduction. Permanent increase of toxic substances in water in sublethal concentrations for hydrobionts for a long time can remain undetected by the nature protection services. It negatively affects the condition of aquatic biological resources. Despite the fact, that lead is a typical thiol poison, pathogenetic mechanisms of the toxic effect of this metal had been described in a fragmentary manner only and require the molecular genetic, cellular and tissue levels study. The aim of the research is to study the peculiarities of organisation of the Siberian sturgeon's stomach and spiral intestine during the experimental modeling of chronic intoxication by lead acetate. Materials and Methods. The object of research was the stomach and spiral intestine of the Siberian sturgeon. All samples were fixed in a 10 per cent formalin solution and carried out according to a standard procedure. The preparations were stained by Böhmer's hematoxylin and eosin. The total acidic glycosaminoglycans were detected by the Stidman method. Collagen distribution in tissues of the stomach and spiral intestine was determined by the Mallory method. Histological specimens were examined in transmitted light using the microscope Axio Imager.M2 (CARL ZEISS, Germany). Results. The Results of the study showed that during the experimental modeling of chronic intoxication by lead acetate, the stomach and spiral intestine are characterized by a differential level of disruption of their structural and functional organization. Conclusions. From the point of view of fundamental biology the authors note, that studying the peculiarities of organisation of the Siberian sturgeon digestive organs using experimental modeling during the process of chronic intoxication by lead acetate allows to clarify the ideas about pathogenetic mechanisms of poisoning and diseases of chemical etiology. This provides a special view on the problem of controlling the detoxification processes in fish.