The influence of an immobilization stress on a structural and functional condition of mast and parafollicular cells of a thyroid gland of a rat

It is known that parafollicular and mast cells share in maintaining of a homeostasis of a thyroid gland. The purpose of the study is to the analysis of influence of an immobilized stress on a morpho-functional condition of parafollicular and mast cells of a thyroid gland of a rat. The study is performed on white not purebred laboratory male rats, which were divided into 2 groups: 1 group - 10 intact animals; the 2nd group - 10 animals subject to an immobilized stress, by their rooming in Kogan's camera. Paraffin cuts of a thyroid gland were painted by 0,1% solution toluidine blue and silver nitrate across Grimelius in Nikonov's modifications. The morpho-functional condition of mast cells and С-cells were estimated by determination of their quantity, subpopulation structure of cages on extent of granular saturation and degranulation, with the subsequent calculation of the index of granular saturation and the index of degranulation. As a result of model of an immobilized stress change of subpopulation structure of both mast and parafollicular cells, increase in the index of their granular saturation, and also decrease in the index of degranulation is received that prove the depressing of functional activity of these cells of a thyroid gland.