Synbiotic agent prototype impact on blood biochemical parameters and calves’ immunological status

The aim of research is to study the biochemical composition of blood serum and indicators of the immune status of calves when included in the feeding ration of a prototype of a synbiotic agent [RU 2020 140 502]. Materials for the study were obtained in the conditions of the SEC Breeding Plant "Vtoraya Pyatiletka" of the Ipatovsk Urban District of the Stavropol Region. Research was carried out in the scientific testing laboratory of the FSBEI HE the Stavropol State Agrarian University, as well as in VNIIOK – a branch of the FSSI "North Caucasian Federal Research Center". The material of the experiment was blood obtained from twelve clinically healthy calves of the red steppe breed, they were disbanded into two groups of six animals (experimental and control), the calves of the experimental group were given a sample of the synbiotic agent. For biochemical analysis, blood was taken from calves of both groups using "monovets" according to the research methods. When studying the biochemical composition of blood se-rum, glucose, modified enzymes (ALT, AST), urea, creatinine, calcium, phosphorus, magnesium, cholesterol, total protein, and protein fractions were determined. To determine the protein fractions, we used the nephelometric method on a KFK 2 photoelectrocolorimeter. BASK was detected on the basis of changes in the optical density of the MPB during the growth of BGKP - Esherichia coli in it. As a result of lysis of the test culture of Micrococcus lysodecticus with blood lysozyme in 0.5% sodium chloride solution, the optical density of the medium changes, due to which we determined LASK. The results obtained indicate a positive shift in the immune status, as well as the qualitative composition of blood serum in calves under the influence of a synbiotic agent, and are characterized by an increase in total protein by 2.5–6.2 % and protein fractions, in particular globulin, by 9.6–13.5 %; BASK – by 5.6 %; LASK – by 0.6 %, which reflects an increased reaction of protein metabolism, improved metabolic processes and, as a result, the immuno-modulatory effect of the developed synbiotic agent.