The effect of plant growth regulator Mival-agro in the nutrient medium on the accelerated development of potatoes in vitro

Relevance. Obtaining high-quality seed potatoes of domestic breeding is a key aspect of Russian potato growing successful development and overcoming dependence on foreign varieties. The use of the clonal micro-propagation method can significantly speed up the production process of high-quality elite seeds. Currently, one of the key factors enhancing the efficiency of clonal micropropagation is the use of plant growth regulators that control morphogenetic processes in culture in vitro. The purpose of the study: to study the effect of various concentrations of the organosilicon plant growth regulator Mival-Agro on the growth and development of healthy potato microplants. Material and methods. Laboratory studies were conducted in the laboratory of microclonal reproduction of Velikiye Luki State Agricultural Academy. The object of research was medium-ripened potato varieties Gusar, Aurora, Manifest, Sirenevyi Tuman and Real. The plant growth regulator Mival-Agro was used as one of the Murashige-Skuga nutrient medium components in concentrations of 2.5 ml/l, 5.0 ml/l and 7.5 ml/l. The experiments were repeated by three times, and 60 test tube plants were studied in each experiment. The data obtained during the experiment were processed by the method of variance analysis. Results. The development level of the root system is a significant factor determining the successful adaptation and subsequent growth of microplants in soil substrate. During the study, it was found that the optimal concentration of Mival-Agro in the nutrient medium for stimulating rhizogenesis was 5 ml/l. By the 21st day of cultivation: the Gusar variety developed 10.5 roots per plant, exceeding the control by 4.2 roots, while root length surpassed the control by 36.2 mm. The Avrora, Sirenevyi Tuman, Real, and Manifest varieties developed 8.1, 8.7, 10.4, and 9.2 roots per plant. Root length in these varieties exceeded the control by 25.4 mm, 30.0 mm, 36.7 mm, and 31.4 mm, respectively.