Influence of nutrition medium composition on root formation of bog blueberry (Vaccinium uliginosum L.) of northern Russian origin in vitro culture

The results of studies on clonal micropropagation of blueberry (Vaccinium uliginosum L.) at the stage of rooting of microshoots in vitro are presented. V. uliginosum is an economically valuable forest berry species in food and medicinal terms, which can be successfully used for cultivation in areas of former peat mining. To quickly obtain a large amount of healthy planting material, it is advisable to use the method of clonal micropropagation. It is necessary to improve the technology for growing V. Uliginosumin vitro culture for forms of Northern Russian origin. The objects of study are plants of V. uliginosum forms Arkhangelsk and Vologda. At the stage of rooting of microshoots in vitro, the highest values of the number of roots (5.2-5.4 pcs.) of V. uliginosum plants were noted on the Woody Plant Medium culture medium. The maximum total length of roots of V. uliginosum of the Arkhangelskaya form (on average 10.6 cm) in vitro culture was detected on the Woody Plant Medium culture medium, the Vologodskaya form (9.3-9.9 cm) - on the Woody Plant Medium and Woody Plant Medium 1/2 media. An increase in the content of indolylacetic acid in the culture medium from 1.0 to 2.0 mg/l contributed to an increase in the number of roots (1.2-1.4 times) and a decrease in the average root length (1.3 times) of V. uliginosum plants of northern Russia origin in vitro culture, while the total length of roots did not differ significantly depending on the form and concentration of auxin.