The effect of the nutrient medium composition on the formation of conidia by G. fusarium fungi

The aim of the study is to investigate the effect of various nutrient media on the production of conidia by the causative agents of wheat fusarium head blight, relevant for the Krasnoyarsk Region. The objects of the study were 11 strains of Fusarium spp., differing in their cultural and morphological characteristics, as well as in the ability to form conidia, which were isolated by the author from the roots and grain of wheat grown at the Borsky Research and Production Complex (56 26′15″ N; 92 54′11″ E) in the forest-steppe zone of the Krasnoyarsk Region, harvested in 2021. The effect of the composition of three nutrient media on the ability of Fusarium fungi to form conidia was studied. Of the three studied media, only the Czapek-Dox medium ensured the formation of conidia in all tested strains, potato sucrose agar is in second place in terms of the ability to induce conidia formation. Medium № 2 GRM (Saburo) contributed minimally to the formation of conidia. The number of conidia per 1 cm2 of culture varied widely depending on the medium and strain. On the Czapek-Dox nutrient medium, the number of macroconidia varied from 66.4 to 1061.9 thousand per 1 cm2, and the number of microconidia - from 149.3 thousand to 2787.4 thousand per 1 cm2, on potato sucrose agar these values were in the range from 199.1 thousand to 1460.1 thousand per 1 cm2 for macroconidia and from 153.5 thousand to 1791.9 thousand per 1 cm2 for microconidia. On medium № 2 GRM (Saburo) for macro- and microconidia, these limits were from 199.1 thousand to 995.5 thousand per 1 cm2 and from 331.8 thousand to 1791.9 thousand per 1 cm2, respectively. For the strains, neither statistically significant general patterns in the influence of the medium composition on the number of formed conidia, nor significant correlations between the number of conidia were revealed.