The exocrine pancreatic function in chicken (Gallus gallus L.) fed diets supplemented with different vegetable oils

The efficiency of different vegetable oils in poultry nutrition is still a matter of interest for the theory and practice; numerous studies in broilers have evidenced that different oils can significantly affect the productive performance and metabolism. However, the mechanism of the influence of different oils on the exocrine pancreatic function is understudied. In our study on fistulated chicken the innovative data are presented for the analysis of the effects of different dietary lipid sources on the secretion and enzymatic activities of pancreatic juice. The study was performed in 2019 on three laying hens ( Gallus gallus L., cross Hisex White) with chronic fistulae of main pancreatic duct inserted by the method of Ts.Zh. Batoev (2001). The physiological trials were performed by the method of periods (7-10 days per period): during the control period the basic feed (commercial compound feed for layers PK-1) was supplemented with sunflower oil; during period 1 with soybean oil; during period 2 rapeseed oil; during period 3 flaxseed oil. The pancreatic juice and blood were sampled throughout these periods. The secretion rate of pancreatic juice and enzymatic activities in it (amylase, lipase, total proteases TP, alkaline phosphatase AP) were determined by standard methods. The activities of amylase, lipase, alanine (ALT) and aspartate (AST) transaminases in blood serum were determined on analyzer ChemWell 2900 (T) (Awareness Technology, USA) with corresponding reagent kits by Human GmbH, Germany; the activities of TP and AP and other biochemical indices in blood serum were determined on semi-automatic analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., China) with BAPNA as the substrate (TP) and corresponding reagent kits by DIAKON-VET, Russia (AP and all other indices). It was found that the activity of lipase in pancreatic juice can adjust to the dietary oil used. The significantly highest lipase activity was found with unrefined sunflower oil (21345±652.8 μmol∕l-1∕min-1, p -1∕min-1, p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }