The introduction to culture in vitro and clonal micropropagation of perspective seedling of black currant (Ribes nigrum L.)

In the Laboratory of Fruit Growing of Russian State Agrarian University-Moscow Timiryazev Agricultural Academy isolated black currant seedling obtained from free pollination and combining optimal complex of economically valuable traits: stably high yield, large-fruit, winter hardiness and resistance to powdery mildew. To accelerate the process of introducing a valuable genotype into production, as well as to obtain healthy plant material with high potential for further vegetative propagation in vivo the main stages of clonal micropropagation of a seedling blackcurrant were developed. To initiate culture the apexes isolated from resting apical and lateral buds and young developing shoots were used. Sterile explants were cultured on nutrition medium. Agarised medium based on mineral elements Murashige and Skoog (1962) (MS), with twice the concentration of iron chelate was used as base medium. Cytokinin - 6-benzylaminopurin (6-BAP) was tested at concentrations of 0.6; 0.7; 0...