Laying the foundations of rare and endangered plant species in vitro genetic bank of the «Sirius» educational center

To conserve rare and endangered plant species, along with the classical methods of ex situ conservation, it is necessary to create in vitro genetic banks. When forming a collection of in vitro plants, one of the important stages of clonal micropropagation is obtaining sterile explants at the injection stage. Seeds were used as explants for rare and endangered plant species. The plant material for the in vitro collection was obtained from the seed bank of the Sirius Children's Botanical Garden. The purpose of research is to create an in vitro genetic bank of rare and endangered plant species on the basis of the Sirius Educational Center. When using this sterilization protocol, the maximum yield of sterile explants was obtained for different plant species, and for some species, seed germination was noted at an early date. For seed germination, a hormone-free medium was chosen according to the prescription of Murashige and Skoog (MS). As a result of the analysis of the obtained data, we have determined an effective protocol for sterilization of seeds in vitro for 14 rare and endangered plant species. At the early stages of the growth and development of explants, seed germination was observed in six species (Maikaragan Volga, Cretaceous resin, Gelman's resin, Fragrant Levkoy, Dioscorea Nippon and Chalk jaundice) plants with a total share of 20-100 %, on the basis of which it is possible to develop methods of clonal micropropagation and preservation of the studied plant species.