A modified D-roms method for determination of lipid peroxidation
Автор: Nassar Muammar A.Y., Sharif Saleh H., Hammad Khalda M.A.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.18, 2022 года.
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The need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative stress a research only biomarker, despite its crucial value as a risk factor and pathologic status, for many inflammatory and metabolic diseases. This work aimed at developing a modified rapid method to determine serum lipid peroxides at wavelength 492 nm and reference wavelength 630 nm. The results showed linear responses with increasing concentration and that the cheap N,N-DM-P-Phenylenediamine diHCl can be used as a chromogen under saving conditions of pH, temperatures and volume of sample.
Determination of lipid peroxides, modified rapid method
Короткий адрес: https://sciup.org/143178656
IDR: 143178656
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