Adaptation of wheat plant-regenerants to ex vitro conditions: stomata functioning

The investigation of leaves stomatal apparatus of wheat regenerants, obtained under conditions of high humidity and low illumination in vitro was carried out during their gradual (15 days period) adaptations to conditions of the lowered air humidity and high illumination ex vitro. It was established, that at the end of experiment regenerants ex vitro and intact plants had similar values of stomatal conductivity. The comparative cytological analysis of stomata of adapted ex vitro regenerants and intact plants in vivo showed their morphological similarity, although the regenerants had smaller sizes of stomata. The offered method of gradual adaptation to ex vitro conditions allows achieving high survival rate of regenerants.