Algorithm of diagnosis of adenomyosis by non-invasive methods

The using of non-invasive methods are offered to early diagnosis of adenomyosis. Comparative MALDI mass spectrometry profiling of blood serum samples from patients with verified adenomyosis (n=120) as well as from a control group of healthy women (n=30) has been carried out. Mass spectrometry profiles demonstrated sensitivity and specificity close to 100% for the detection of adenomyosis. On the second stage we discovered the production of cytokines (IL-6, IL-10) and growth factors (EGF, VEGF) by an enzyme-linked immunosorbent assay from women with adenomiosis. We observed that levels of IL-6, IL-10, EGF, VEGF are correlated with the severity of the disease and prognosis.