Allelopathic stress produced by bitter gourd ( Momordica charantia L.)

Allelopathic stress produced by bitter gourd ( Momordica charantia L.)

Автор: Singh N.B., Sunaina

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 2 т.10, 2014 года.

Бесплатный доступ

The present study deals with in vitro effects of allelochemicals present in leaf and fruit leachate of Momordica charantia in vitro on plant growth and metabolism of Lycopersicon esculentum. Momordica was selected as a donor plant and tomato as recipient. Seeds of tomato were shown in pots and after germination different concentrations viz. 25, 50, 75 and 100% of leaf and fruit leachates were applied as treatment. Twenty days old seedlings were harvested for biophysical and biochemical analyses. The root and shoot length, fresh and dry weight of the seedlings decreased in dose dependent manner. The reduction in pigment and protein contents and nitrate reductase activity was concentration dependent. Membrane leakage increased as the concentration of leachates increased. Activities of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) activities significantly enhanced under allelopathic stress. Inhibition of various metabolic activities under allelopathic stress resulted in decreased plant growth and development. The fruit leachate of Momordica was more inhibitory than leaf leachate.

Еще

Allelopathy, antioxidants, electrolyte leakage, leachates, momordica

Короткий адрес: https://sciup.org/14323875

IDR: 14323875

Список литературы Allelopathic stress produced by bitter gourd ( Momordica charantia L.)

  • Bagavathy S. and Xavier G.S.A. (2007) Effects of aqueous extract of Eucalyptus globulus on germination and seedling growth of sorghum. Allelopathy J., 20(2), 395-402
  • Bais H.P., Vepechedu R., Gilroy S., Callaway R.M. and Vivanco J.M. (2003) Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science, 301, 1377-1380
  • Barkosky R.R. and Einhellig F.A. (2003) Allelopathic interference of plant water relationships by para hydoxybenzoic acid. Bot Bull. Acad. Sin., 44, 53-58
  • Barkosky R.R., Butler J.L. and Einhellig F.A. (2000) Caffeic acid-induced changes in plant water relationships and photosynthesis in leafy spurge. J Chem. Ecol., 26(9), 2095-2109
  • Batish D.R., Singh H.P., Rana N. and Kohli R.K. (2006) Assessment of allelopathic interference of Chenopodium album through its leachates, debris extracts, rhizosphere and amended soil. Archives of Agr. and Soil Sci., 52, 705-715
  • Beauchamp C. and Fridovich I. (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44, 276-287
  • Cakmak I. and Marschner H. (1992) Magnesium deficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol., 98, 1222-1227
  • Cruz-Ortega R., Ayala-Cordero G. and Anaya A.L. (2002) Allelochemical stress produced by the aqueous leachates of Calicarpa acuminate: effects on roots of bean, maize and tomato. Physiol Plant, 116, 20-27
  • Doblinski P.M.F., Ferrarese M.L.L., Huber D.A., Scapim C.A., Braccini A.L. and Ferrarese-Filho O. (2003) Peroxidase and lipid peroxidation of soybean roots in response to p-Coumaric and p-Hydroxybenzoic acids. Braz. Arch. Biol. Tech., 46(2), 193-198
  • Galindo J.C.G., Hernandez A., Dayan F.E., Te˜llez M.R., Macias F.A, Paul R.N. and Duke S.O. (1999) Dehydrozaluzanin C, a natural sesquiterpenolide, causes rapid plasma membrane leakage. Phytochem., 52, 805-813
  • Gomez J.M., Jimenez A., Olmos E. and Seveilla F. (2004). Location and effects of long term NaCl stress on superoxide dismutase and ascorbate peroxidase isoenzymes of pea (Pisum sativum cv. Puget) chloroplast. J of Experi. Bot., 55, 119-130
  • Heath R.L. and Packer L. (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125, 189-198
  • Hedge J.E. and Hofreiter B.T. (1962) Estimation of carbohydrate. In:Whistler, R.L. and Be Miller, J.N. (ed.), Methods in carbohydrate chemistry. Academic Press, New York, pp. 17-22
  • Jaworski E. (1971) Nitrate reductase assay in intact plant tissue. Biochem. Biophys. Res. Commun., 430, 1274-1279
  • Kaiser W.M., Spill D. and Glaab J. (1993). Rapid modulation of Nitrate reductase in leaf and roots: Indirect evidence for the involvement of protein phosphorylation/dephosphorylation. Physiol. Planta., 89, 557-562
  • Lichtenthaler H.K. (1987) Chlorophyll and carotenoids: pigments of photosynthetic bio-membranes. In: Packer L, Douce R, editors. Methods Enzymology. Academic Press, Sandiego, pp., 350-382
  • Lowry O.H., Rosenbrough R.J., Farr A.L. and Randall R.J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem., 193, 265-275
  • Nürnberger T., Nennstiel D., Jabs T., Sacks W.R., Hahlbrock K. and Scheel D. (1994). High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defence responses. Cell, 78, 449-460
  • Rice E.L. (1984) Allelopathy, 2nd edn. Academic Press, Orlando
  • Rubio M.C., Gonzalez E.M., Minchin F.R., Webb K.J., Arrese-Igor C., Ramos J. and Becana M. (2002) Effect of water stress on antioxidant enzymes of leaves and nodules of transgenic alfalfa over expressing superoxide dismutases. Physiol. Planta., 115, 531-540
  • Singh A., Singh D. and Singh N.B. (2009). Allelochemical stress produced by aqueous leachate of Nicotiana plumbaginifolia Viv. Plant Growth Regul., 58,163-171
  • Singh N.B., Pandey B.N. and Singh A. (2009). Allelopathic effects of Cyperus rotundus extract in vitro and ex vitro on banana. Acta Physiol. Planta., 31, 633-638
  • Singh N.B., Singh A. and Singh D. (2008) Autotoxic effects of Lycopersicon esculentum. Allelopathy J, 22(2), 429-442
  • Singh U.P., Maurya S., Singh A. and Singh M. (2011) Phenolic acid in some Indian cultivars of Momordica charantia and their therapeutic properties. J of Medi. Plants Res., 5(15), 3558-3560
  • Thapar R. and Singh N.B. (2006). Phytotoxic effects of Cassia tora on growth and metabolism of Parthenium hysterophorus L. Allelopathy J, 17, 235-246
  • Unyayar S., Keles Y.C. Cekic F.O. (2005) The antioxidative response of two tomato species with different drought tolerances as a result of drought and cadmium stress combinations. Plant Soil Environ., 51(2), 57-64
  • Venkateshwarlu G, Ravindra V, Prabha C. (2001) Mangiferin: an allelopathin from mango (Mangifera indica L.) leaves. Allelopathy J, 8, 221-224
  • Weir T.L., Park S.W. and Vivanco J.M. (2004) Biochemical and physiological mechanisms mediated by allelochemicals. Curr Opin Plant Biol., 7, 472-479
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp