Alleviation of the effects of NaCl salinity in spinach ( Spinacia oleracea L. var. All Green) using plant growth regulators

Автор: Ratnakar Anjali, Rai Aruna

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 3 т.9, 2013 года.

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Exposure to environmental stress due to salinity has been reported to result in adverse effects on the growth of plants. Studies have shown that the use of plant growth regulators (PGRs) has an ameliorative effect on plants grown under saline conditions. In the present investigation, effect of gibberellic acid (GA 3), 6-furfuryladenine (Kinetin) and benzyl adenine (BA) on Spinacia oleracea L. var. All Green, cultivated under saline conditions has been studied. After a pre-soaking treatment of six hours in 20 mg L -1 solutions of GA 3, Kinetin and BA, the seeds were allowed to germinate and grow for forty-five days under saline conditions. On the analysis of mature leaves, it was observed that both chlorophyll a and b, and total chlorophyll showed an increase in PGR-treated plants compared to the untreated set. With the pretreatment, the reducing and non-reducing sugar content, as well as protein content of the leaves showed an increase in accumulation compared to the untreated plants. The accumulation of the stress metabolite proline, which increases under saline conditions, showed a significant decrease in the plants pretreated with PGRs.

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Benzyle adenine, gibberellic acid, kinein, plant growth regulators, salinity

Короткий адрес: https://sciup.org/14323758

IDR: 14323758

Список литературы Alleviation of the effects of NaCl salinity in spinach ( Spinacia oleracea L. var. All Green) using plant growth regulators

  • Afzal, I., Basra, S.M.A. and Iqbal, A. (2005): The effects of seed soaking with plant growth regulators on seedling vigor of wheat under salinity stress. Journal of Stress Physiology and Biochemistry, 1(1): 06-14.
  • Ali, H.M., Siddiqui, M.H., Basalah, M.O., Al-Whaibi, M.H., Sakran, A.M. and Al-Amri, A. (2012): Effects of gibberellic acid on growth and photosynthetic pigments of Hibiscus sabdariffa L. under salt stress. African Journal of Biotechnology, 11(4): 800-804.
  • Almodares, A., Hadi, M.R. and Dosti, B. (2008): The effects of salt stress on growth parameters and carbohydrates contents in sweet sorghum. Research Journal of Environmental Sciences, 2(4): 298-304.
  • Alsokari, S.S. (2009): Modulatory role of kinetin on photosynthetic characteristics, yield and yield attributes of cadmium-treated Sorghum bicolor plants. Journal of Applied Sciences Research, 5(12): 2383-2396.
  • Amirjani, M.R. (2010): Effect of NaCl on some physiological parameters of rice. EJBS, 3(1): 06-16.
  • Arnon, D.I. (1949): Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol., 24: 1-15.
  • Arulbalachandran, D., Sankar Ganesh, K. and Subramani, A. (2009): Changes in metabolites and antioxidant enzyme activity of three Vigna species induced by NaCl stress. American-Eurasian Journal of Agronomy, 2(2): 109-116.
  • Bagdi, D.L. and Afria, B.S. (2008): Alleviation of salinity effects using plant growth regulators in wheat. Indian Journal of Plant Physiology, 13(3): 272-277.
  • Bates, L.S., Waldren, R.P. and Teary, I.D. (1973): Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207.
  • Chauhan, J.S., Tomar, Y.K., Singh, N.I., Ali, S. and Debarati (2009): Effect of growth hormones on seed germination and seedling growth of black gram and horse gram. Journal of American Science, 5(5): 79-84.
  • Chookhampaeng, S. (2011): The effect of salt stress on growth, chlorophyll content, proline content and antioxidative enzymes of pepper (Capsicum annuum L.) seedling. European Journal of Scientific Research, 49(1): 103-109.
  • Doganlar, Z.B., Demir, K., Basak, H. and Gul, I. (2010): Effects of salt stress on pigment and total soluble protein contents of three different tomato cultivars. African Journal of Agricultural Research, 5(15): 2056-2065.
  • El Sayed, H.S.A. (2011): Influence of salinity (NaCl and Na2SO4) treatments on growth development of broad bean (Vicia faba L.) plant. American-Eurasian Journal of Agricultural and Environmental Sciences, 10(4): 600-610.
  • Jaleel, C.A., Sankar, B., Sridharan, R. and Paneerselvam, R. (2008): Soil salinity alters growth, chlorophyll content and secondary metabolite accumulation in Catharanthus roseus. Turk Journal of Biology, 32: 79-83.
  • Jat, N.K. and Sharma, V. (2006): The interactive effect of salinity and PGR on certain bio-chemical parameters in wheat seedlings. American Journal of Plant Physiology, 1(2): 132-141.
  • Kasim, W.A. and Dowidar, S. (2006): Amino acids and soluble protein profile of radish seedlings under salt stress as affected by GA3 priming. Indian Journal of Plant Physiology, 11(1): 75-82.
  • Kaya, C., Higgs, D. and Kirnak, H. (2001): The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Bulgarian Journal of Plant Physiology, 27(3-4): 47-59.
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951): Protein measurement with Folin Reagent. Journal of Biological Chemistry, 93: 265-275.
  • Mahajan, S. and Tuteja, N. (2005): Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444: 139-158.
  • Mohammad, A.H.M.A. (2007): Physiological aspects of mungbean plant (Vigna radiata L.) in response to salt stress and gibberellic acid treatment. Research Journal of Agriculture and Biological Sciences, 3(4): 200-213.
  • Mezni, M., Albouchi, A., Bizid, E. and Hamza, M. (2010): Minerals uptake, organic osmotica contents and water balance in Alfalfa under salt stress. Journal of Phytology, 2(11): 01-12.
  • Miller, G.L. (1959): Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3): 426-428.
  • Munns, R. (1993): Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses. Plant Cell and Environment, 16: 15-24.
  • Niazi, B.H., Rozema, J. and Athar, M. (2005): Effect of pre-germination and post-germination treatment with growth hormones (kinetin and abscisic acid) on ion concentration and biochemical contents of fodderbeet and seebeet under saline conditions. General and Applied Physiology, 31(1-2): 89-104.
  • Patel, A.D., Panchal, N.S., Pandey, I.B. and Pandey, A.N. (2010): Growth, water status and nutrient accumulation of seedlings of Jatropha curcas L. (Euphorbiaceae) in response to salinity. Anales de Biologia, 32: 59-71.
  • Sarwat, M.I. and El-Sherif, M. (2007): Increasing salt tolerance in some barley genotypes (Hordeum vulgare) by using kinetin and benzyladenine. World Journal of Agricultural Sciences, 3(5): 617-629.
  • Shomeili, M., Nabipour, M., Meskarbashee, M. and Memari, H.R. (2011): Effects of gibberellic acid on sugarcane plants exposed to salinity under a hydroponic system. African Journal of Plant Science, 5(10): 609-616.
  • Siddiqui, M.H., Mohammad, F., Khan, M.N., Al-Wahibi, M.H. and Bahkli, A.H.A. (2010); Nitrogen in relation to photosynthetic capacity and accumulation of osmoprotectant and nutrients in Brassica genotypes grown under salt stress. Agricultural Sciences in China, 9(5): 671-680.
  • Siringam, K., Juntawong, N., Cha-Um, S. and Kirdmanee, C. (2011): Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under iso-osmotic conditions. African Journal of Biotechnology, 10(8): 1340-1346.
  • Tammam, A.A., Abou Alhamd, M.F. and Hemeda, M.M. (2008): Study of salt tolerance in wheat (Triticum aestivum L.) cultivar Banysoif 1. Australian Journal of Crop Science, 1(3): 115-125.
  • Zeid, I.M. (2011): Alleviation of seawater stress during germination and early growth of barley. International Journal of Agriculture: Research and Review, 1(2): 59-67.
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