Alterations of protein and DNA profiles of Zea mays L. under UV- B radiation
Автор: John De britto A., Jeevitha M., Leon Stephan raj T.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 4 т.7, 2011 года.
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UV radiation is an important stress factor for plants which may result in damage to genetic system and cell membranes and several metabolic processes. UV-B has greater damaging effects on plants because the cell macromolecules such as DNA and protein having strong absorption at 280-320 nm. In the present study, UV-B stress was given to the seeds of ZeamaysL. two different time intervals (30 and 60 min) and that stressed seeds were grown under normal environment condition. The leaves of 10thand 20thday seedlings were collected for the analysis of protein and DNA profiles. Protein was analyzed by SDS-PAGE and DNA was analyzed by Restriction enzymes. When compared with control plants, increased numbers of protein and DNA bands were observed in UV-B treated plants. The present study concluded that the plant synthesis new proteins and DNA under UV treatment for the adaptation to the environmental conditions. These stressed proteins could be used as biomarkers for identification of stressed plant. Identification of quantitative trait loci for UV stress resistance may well be an effective analytical tool. This approach is promising, considering that saturated DNA marker maps are now available for both genetic model plants and crop plants.
Restriction enzymes, sds-page, stress response, uv-b radiation, zea mays l.
Короткий адрес: https://sciup.org/14323554
IDR: 14323554
Список литературы Alterations of protein and DNA profiles of Zea mays L. under UV- B radiation
- Britt, A. B. (1996) DNA damage and repair in plants. Ann. Rev. Plant Physiol. Plant Mole. Biol. 47: 75-100.
- Britt, A. B. (1997) Genetic analysis of DNA repair in plants. In Lumsden P Mpoloka 4881(ed.). Plants and UV-B: Responses to environmental change, Cambridge University Press. pp 77-93.
- Caldwell, M. M., Teramura, A. H., Tevini, M. (1989) The changing solar ultraviolet climate and the ecological consequences for higher plants. TREE, 4: 363-367.
- Casati, P. and Walbot, V. (2004) Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues. Genome Biol. 5, R16.
- Casati, P., Zhang, X., Burlingame, A. L. and Walbot, V. (2005) of Leaf Proteome after UV-B in Maize Lines Differing in. Molecular & Cellular Proteomics, 4:1673-1685.
- Charles, M.T., Tano, K., Asselin, A. and Arul, J. (2008) Physiological basis of UV-C induced resistance to Botrytis cinerea in tomato fruit V. Constitutive defence enzymes and inducible pathogenesis-related proteins.
- Doyle, J. J. and Doyle, J. L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bul., 19: 11-15.
- Gerhard, R., Werner, H., Holger, P., Heinrich, S., Harald, K.S. and Barbara, H. (2000) Elevated UV-B radiation reduces genome stability in plants. Nature, 406: 98-101.
- Jordan, B. R. (2002) Molecular response of plant cells to UV-B stress. Functional Plant Biol. 29: 909-916.
- Jordan, B. R., He, J., Chow, W. S. and Anderson, J. M. (1992) Changes in mRNA levels and polypeptide subunits of ribulose 1,5-bisphosphate carboxylase in response to supplemental UV-B radiation. Plant, Cell and Environment, 15: 91-8.
- Laemmli, U. K. (1970) Cleavage of structure proteins assembly of the head of bacteriophage. T4. Nature, 22: 680-5.
- Lowry, O. H., Roseberough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.
- Longstreth, J. D., de Gruijl, F. R., Kripke, M. L., Takizawa, Y. and van der Leun, J. C. (1995) Effects of increased solar ultraviolet radiation on human health. Ambio, 24: 153-165.
- Mitchell, D. L. and Nairn, R. S. (1989) The biology of the (6-4) photoproduct. Photochem. Photobiol., 49: 805-819.
- Mpoloka, S.W. (2008) Investigating Evidence for UV-B Induced Mutagenesis in Dimorphotheca sinuata Using the 18S rDNA and rbcL Gene Sequences, Adv. Environ. Biol., 2(2): 81-88.
- Musil, C. F., Chimphango, S. B. M., Dakora, F. D. (2002) Effects of elevated ultraviolet-B radiation on native and cultivated plants of southern Africa. Anna. Bot.90: 127-137.
- Musil, C. F. (1996) Cumulative effect of elevated ultraviolet-B radiation over three generations of the arid environment ephemeral Dimorphotheca sinuata (Asteraceae). Plant Cell Environ.19: 1017-1027.
- Paula, C. and Virginia, W. (2004) Crosslinking of ribosomal proteins to RNA in maize ribosomes by UV-B and its effects on translation. Plant physiology, 136(2): 3319-3332.
- Rozema, J., Staaij van de, J., Bjorn, O. L., Caldwell, M. (1997) UV-B as an environmental factor in plant life: stress and regulation. Trends Ecol. Evol.12(1): 22-28.
- Rozema, J., Bjorn, O. L., Bornman, J. F. (2002) The role of UV-B radiation in aquatic and terrestrial ecosystems -an experimental and functional analysis of the evolution of UV-B absorbing compounds. J. Photochem. Photobiol. B: Biol.66: 2-12.
- Sambrook and Russelle. (2000) Molecular cloning: A labortary manual. Third edition. Vol:1 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, Newyork, USA.
- Sancar, A. and Tang, M. S. (1993) Photobiology school:nucleotide excision repair. Photochem. Photobiol. 57: 905-921.
- Strid, A., Chow, W. S., Anderson, J. M. (1994) UV-B damage and protection at the molecular level in plants. Photosynth. Res.39: 475-489.
- Taylor, R. M., Tobin, A. K., Bray, C. M. (1997) DNA damage and repair in plants. Plants and UV-B: Responses to environmental change. Edited by Lumsden P, Cambridge University Press.
- Zhang, J. E., Hu, X., Henkow, L., Jordan, B. R. and Strid, A. 1994. The effects of ultraviolet-B radiation on the CFoF,-ATPASE. Biochimica Biophysica Ada 1185: 295-302.
- Zuk-Golaszewska, K., Upadhyaya, M. K., Golaswewski, J. (2003) The effect of UV-B radiation on plant growth and development. Plant Soil Environ. 49: 135-140.
