An appraisal on the stress amelioration of effluent treated vetiver plants amended with ascorbic acid in constructed wetlands

An appraisal on the stress amelioration of effluent treated vetiver plants amended with ascorbic acid in constructed wetlands

Автор: Dhanya G., Vinod Gopal V., Jaya D.S.

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 1 т.18, 2022 года.

Бесплатный доступ

Industrial effluents are one of the major sources of direct and continuous input of pollutants in water bodies. It is the waste generated by manufacturing or industrial processes and it include wide variety of pollutants including harmful heavy metals. Therefore, water treatment is unavoidable though it is very expensive and very hard to carry out. Chemical treatments and electro-kinetics have been resulting in rendering the soil biologically dead and useless for plant growth. Therefore, vegetative methods (phytoremediation using hyper-accumulator plants) are thought to be most practical and economical. The study was conducted to find out the physiological and biochemical changes in Vetiver grass ( Vetiveria zizanioides (L.) Nash.) treated with service station effluent in constructed wetlands, and to assess the phytoremediation potential of it. The study also aimed to evaluate the effect of ascorbic acid treatment on the phytoremediation potential and biochemical changes of the wastewater stressed vetiver plants. In the study, ninety days old vetiver plants grown in constructed wetlands were treated with 15 L of automobile service station effluent for 15 days. Another test group plants were treated with the effluent along with ascorbic acid (10 mg ml -1) on alternate days for the 15 days. After the experimental period, treated effluents and the plant parts of the control and test groups were collected, and various chemical and biochemical attributes were determined following the standard procedures. Results of the study showed that more than 50% removal of pollutants was achieved after 15 days treatment in constructed wetlands. The biochemical analysis of treated plant parts showed decrease in the concentration of pigments, biomolecules, total phenols and nutrients. Also there was increase in the concentration of antioxidants and malondialdehyde. The study also revealed that the ascorbic acid addition to the constructed wetland system increased the concentration of pigments and biomolecules in the test plants. Ascorbic acid amendment enhanced the defence mechanism against the wastewater induced oxidative stress and thereby increased the pollutant removal efficiency of vetiver.

Еще

Ascorbic acid, antioxidants, constructed wetland, phytoremediation, service station effluent, vetiver

Короткий адрес: https://sciup.org/143178341

IDR: 143178341

Список литературы An appraisal on the stress amelioration of effluent treated vetiver plants amended with ascorbic acid in constructed wetlands

  • Al-Hakimi A. B.M. and Hamada A.M. (2011). Ascorbic acid, thiamine or salicylic acid induced changes in some physiological parameters in wheat grown under copper stress. Plant Prot. Sci. 47, 92-108.
  • Annonymous. (2009). Eco-Friendly and Non Energy Intensive Technologies for Waste Water Treatment, Eco-Housing Assessment Criteria - Version II, Implemented under Eco-housing Mainstreaming Partnership by IIEC with funding support from USAID, Annexure 6, 1-12.
  • APHA. (2012). Standard Methods for the Examination of Water and Wastewater, 22nd ed., APHA- AWWA-WEF. Washington, DC.
  • Balakhnina, T.K., Kosohryukhov, A.A., Ivanov, A.A. and Kreslavskil. V., D. (2004). Toxic responses of Cd in higher plants. J. Environ. Biol. 13, 214-215.
  • Billore, S. K., Ram, H., Singh, N., Thomas, R., Nelson, R. M. and Pare, B. (2002). Treatment performance Evaluation of Surfactant removal from domestic wastewater in a tropical horizontal subsurface constructed wetland. In: Proceedings of the International Conference on Wetland systems for water pollution control, Dar-es Salaam, Tanzania. 16-19.
  • Conklin, P.L. (2001). Recent advances in the role and biosynthesis of ASA in plants. Plant Cell Environ. 24, 383-394.
  • Devi, S. R. and Prasad M. N. V. (2005). Antioxidant capacity of Bracssica juncea plant exposed to elevated levels of copper. Russ. J. Plant Physiol. 52, 205- 208.
  • Dhanya, G. and Jaya, D.S. (2011). Evaluation of Growth Response and Oxidative Stress Biomarkers in Vetiver grass used for the Remediation of Contaminated Soils. Res. J.Chem. Environ. 15 (2), 1-9.
  • Dhanya,G., Jaya, D.S. 2012. Protective effect of ascorbic acid on cadmium induced toxicity in cluster bean Cyamopsis tetragonoloba. L. Taub plants. The Ecoscan, 6(3), 119-124.
  • Dietz, K.-J., Kr€amer, U. and Baier, M. (1999). Free radicals and reactive oxygen species ROS as mediators of damage during heavy metal stress. In: Prasad, M.N.V., Hagemeyer,J. Eds., Heavy Metal Stress in Plants: Molecules to Ecosystem. SpringerVerlag, 73-97.
  • EPA (2002). The Environment Protection Act- 2002. Standards for Effluent Discharge Regulations. General Notice. No. 44 of 2003.
  • Ezhili, N. Manikandan, R. and Venkatachalam, P. (2012). Effects of phosphorus on physiological, photosynthetic pigments and oxidative status in Vetiver grass (Vetiveria zizanioides L). under lead Pb treatment. In: Proceedings of International Conference on Biotechnology, Biological and Biosystems Engineering (ICBBBE 2012) December 18-19, 2012 Phuket Thailand.
  • Graham, P. H. and Vance, C. P. (2003). Legumes: importance and constraints to greater use. Plant Physiol. 131 (3), 872-877.
  • Gupta, P.K. (1999). Soil, plant, water and fertilizer analysis, Agro Botanica Publishers and distributors, Bikaner, India, pp. 438.
  • Hoffmann, H., Platzer, I,C., Winker, I. M. and Muench, E. (2011). Technology review of constructed wetlands-Subsurface flow constructed wetlands for greywater and domestic wastewater treatment. Internationale Zusammenarbeit (GIZ) GmbH Sustainable sanitation - Ecosan program, Eschborn, Germany.
  • Imevbore, A.A.A. and Adeyemi, S.A. (1981). Environmental monitoring in relation to pollution and control of oil pollution. In: Proceedings of the seminar on the petroleum industry and the Nigerian environment, 6, 135-142.
  • Khan, T. A., Mazid, M. and Mohammad, F. (2011). A review of ascorbic acid potentialities against oxidative stress induced in plants, J. Agrobiol. 28(2), 97-111.
  • Koottatep, T., Polprasert, C. and Oanh, N. K. (2010). Design considerations of constructed wetlands for Septage treatment at the ait pilot plant. Asian Institute of Technology Bangkok, Thailand - Urban Environment, Engineering & Managament Program.
  • Luna, C. M., Gonzalez,V. S. and Trippi,V. S. (1994). Oxidative damage caused by excess Copper in Oat leaves. Plant Cell. Physiol. 35(1), 11-15.
  • Matysik, J., Alia, Bhalu, B. and Mohanty, P. (2002). Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr.Sci. 82(5), 525-532.
  • Meloni Da, Oliva Ma, Martinez Ca and Cambraia, J. (2003). Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ. Exper. Bot. 49, 69-76.
  • Nagajyoti, P.C., Dinakar, N., Suresh, S.,Udaykiran, Y., Suresh,C. and Damodharam, T. (2009). Effect of industrial effluent on the morphological parameters and chlorophyll content of green gram (Phaseolus aureus Roxb.). J. Exp. Biol. 303, 385-388.
  • Navari-Izzo, F., Quartacci, M.F. and Izzo, R. (1990). Water-Stress Induced Changes in Protein and Free Amino Acids in Field-Grown Maize and Sunflower. Plant Physiol. Biochem. 28:531-537.
  • Nishikimi, M. and Yagi, K. (1996). Biochemistry and molecular biology of ascorbic acid biosynthesis. Subcell. Biochem. 25, 17-39.
  • Ozturk, L.K., Eker, S. and Ozku Hu. (2003). Effect of Cd on growth and concentrations of Cd, ASA and sal phydryl groups in Drum wheat cultivars. Turk. J. Agric. For. 27, 161-168.
  • Pandey.S., Gupta, K. and Mukharjee, A.K. (2007). Impact of Cadmium and Pb on Catheranthus roseas - A phytoremediation study. J. Environ. Biol. 28(3), 655-662.
  • Raman N.S. and Devotte, S. (2006). Hand book of Indian Environmental Standards. National Environmental and Engineering Research Institute, Nagpur.
  • Reed, S. C., Crites, R. W. and Middle Brookes, E. J. (1995). Natural systems for waste management and Treatment. Mc. Graw. Hill Inc., 430.
  • Sadasivam, S. and Manickam, A. (1996). Biochemical methods. New Age International Publishers, New Delhi, 110.
  • Shaddad, L.M.A., Radi, A.F., Abdul-Rahman, A.M. and Azooz, A. (1990). Response of seeds of Lupines termis and Vicia faba to interactive effect of salinity and ascorbic acid or pyridoxine. Plant Soil. 122, 177-183.
  • Shivhare, N. and Roy, M. (2013). Gravel Bed Constructed wetland for treatment of Sewage water. Poll. Res. 32(2), 415-419.
  • Starlin, T. and Gopalakrishnan, V. (2013). Enzymatic and Non-Enzymatic Antioxidant Properties of Tylophora Pauciflora Wight and Arn. - An In Vitro Study. Asian. J. Pharm. Clin. Res. 6(4), 69-71.
  • Taiz, L. and Zeiger, E. (2002). Plant Physiology (3rd ed.). Sunderland, MA: Sinuaer Associates, Inc.
  • Talaat, N.B. (2003). Physiological studies on the effect of salinity, ascorbic acid and Putrescine of sweet pepper plant. Ph.D. Thesis, Faculty of Agriculture, Cairo University.
  • Tarraf, S.A., El-Din, K.M.G. and Balbaa, L.K. (1999). The response of vegetative growth, essential oil of lemmon grass to foliar application of ascorbic acid, microtinamid and some micro nutrients. Arab Univ. J. Agric. Sci.7, 247-249.
  • Thamizhiniyan, P. Murthy, S.P. and Lakshmanachari, A. S. (2000). Effect of sugar mill effluent and their effects on germination, growth and pigment contents of ground nut and paddy. Ind. J. App. Pure Biol. 15, 151-155.
  • Trivedy, R.K. and Goel, P.K. (1998). Practical methods in Ecology and Environmental Science. Environmedia Publications. India.
  • Truong, P., Mason, F., Waters, D. and Moody, P. (2000). Application of Vetiver Grass Technology in off-site pollution control: Trapping agrochemicals and nutrients in agricultural lands. In: Proceedings of Second International Vetiver Conference, Thailand, January 2000.
  • UN-HABITAT. (2008). Constructed Wetlands Manual. UN-HABITAT Water for Asian Cities Programme, Nepal, Kathmandu, pp. 90.
  • West, L. (2006). World Water Day: A Billion People Worldwide Lack Safe Drinking Water. In: http://environment.about.com/od/environmentaleven ts/a/waterdayqa.htm.
  • Vipat, V., Singh, U. R. and Billore, S. K. (2008). Efficacy of Root Zone Technology for Treatment of Domestic wastewater: Field scale study of a pilot project in Bhopal (MP), India. In: Proceedings of Taal 2007: The 12th World Lake Conference, 995-1003.
  • Zengin, F, K. and Kirbag, S. (2007). Effects of Cu on chlorophyll, Proline, protein and abscisic and level of sunflower seedlings. J. Environ. Biol. 28(3), 561566.
  • Zhang,W., Tian, Z., Pan, X., Zhao, X. and Wang F. (2013). Oxidative Stress and Non-enzymatic Antioxidants in Leaves of Three Edible Canna Cultivars under Drought Stress. Environ. Biotechnol. 54(1), 1-8.
  • Zhu, Y.I., Elizebeth, A.H., Smith P. and Jerry, J.N. (1999). Overexpression of Glutathione Synthetase in Indian Mustard Enhances Cadmium Accumulation and Tolerance. Plant Physiol. 19, 7380.
  • Zirschky, J. (1986). Basic Design Rationale for Artificial Wetland, Contract Report 68-01-7108, US EPA, Office of Muncipal Pollution Control.
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp