Analysis of pathohistological, histochemical, immunohistochemical changes in the liver of rats in dynamics with an experimentally reproduced model of primary hypothyroidism

An experiment was carried out on 86 white male rats of the "Wistar" line with the reproduction of a model of primary hypothyroidism by surgical removal of the thyroid glands. Pieces of the liver were selected for histological examination with their subsequent fixation in 10% buffered neutral formalin. The immunohistochemical method showed an increase in the positive expression of caspase-3 on the twenty-first to twenty-eighth days of the experiment and a decrease in Bcl-2 expression starting from the twenty-first day, an increase in the proliferative activity index according to Ki67 expression on the twenty-eighth day, with the beginning of its decrease by the end of the forty-fifth days of experience.