Endogenous and exogenous factors' influence analysis on blood cells functional activity in bulls

Research objective is to study the dependence of the functional activity (FA) of antigen-activated in vitro blood cells of bulls on endogenous and exogenous factors. The study revealed significant factors influencing the generation of reactive oxygen species (ROS) by blood cells; established the parameters of these factors’ influence. The blood of clinically healthy sperm donor bulls of JSC "Krasnoyarskgosplem" of different ages in different seasonal periods was studied. FA of cells was assessed by the kinetics of ROS generation, recorded by the micro-method of luminol-enhanced chemiluminescence using the hardware-software complex Chemiluminometer 3604-PC (SKTB Nauka SB RAS). It was found that FA of blood cells of bulls-producers has two-phase kinetics and is characterized by regular age-related dynamics and seasonal dependence. The time to reach the first and second maxima of the chemiluminescence curve of the kinetics of ROS generation in clinically healthy bulls is constant. The ratio of the amplitudes of the maxima of the kinetics of ROS generation depends on the season, while the parameters of the first maximum (Imax1) change insignificantly. The smallest Imax1 value was recorded in bulls aged 49–60 months (726 ± 73 counts/s) in the spring and the highest at the age of 26–36 months. (1795 ± 208 counts/s) in the autumn. The fluctuations in the values of the second maximum (Imax2) from (1133 ± 115 counts/s) were revealed in the age group of 19–24 months in autumn up to (3887 ± 525 imp / s) in groups older than 61 months in spring, with a general tendency to decrease the value of both peaks at the age of 37–48 months. The volume (S) of generated ROS increases by the age of 5–6 years, depending on the season (higher in winter). In the component analysis of the kinetics of ROS generation by blood cells, reflecting the participation of cellular and enzyme structures according to the parameters of the model integral chemiluminescence curve (according to Magrisso M.Y. et al., 2000), the main functional states of cells were assessed: spring – the state of "rest"; autumn and winter – the state of "alternative activation". An increase in the integral volume of production of all types of ROS is determined by an increase in the maximum intensity and volume of the third component of the generation of free oxygen radicals, which is not associated with the process of phagocytosis.