Antioxidative defense responses to lead-induced oxidative stress in Glycine max L. cv. Merrill grown in different ph gradient

Antioxidative defense responses to lead-induced oxidative stress in Glycine max L. cv. Merrill grown in different ph gradient

Автор: Mishra Pankaj Kishor, Kumar Umesh, Mishra Meenakshi, Prakash Veeru

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 2 т.9, 2013 года.

Бесплатный доступ

Physiological and biochemical changes as well as the activities of anti-oxidative enzymes under lead (Pb 2+) phytotoxicity were investigated in 20 days old soybean ( Glycine max L.) seedlings grown hydroponically in the laboratory under different pH conditions. The rapid uptake of Pb 2+ was observed immediately after the start of treatment. The quantity of accumulation of Pb 2+ was much higher in roots than in shoots, its level rising with increasing pH from 3.0 to 8.0. Not only that, an oxidative stress conditions were observed due to increased level of superoxide anion radical and hydrogen peroxide in shoots and root cells of 20 days old seedlings when treated with Pb(NO 3) 2 at a concentration of 0, 500, 1000 and 2000 μM. Spectrometric assays of seedlings showed increased level of activities of antioxidant enzymes like catalase, peroxidase and glutathione reductase. The presence of thiobarbituric acid reacting substances (TBARS) indicates the enhanced lipid peroxidation compared to controls. The alteration in the activities of the antioxidant enzymes and the induction of lipid peroxidation reflects the presence of Pb 2+, which may cause oxidative stress.

Еще

Antioxidant enzymes, catalase, free radicals, lipid peroxidation, oxidative stress, superoxide dismutase

Короткий адрес: https://sciup.org/14323730

IDR: 14323730

Список литературы Antioxidative defense responses to lead-induced oxidative stress in Glycine max L. cv. Merrill grown in different ph gradient

  • Ahmad, M.S.A., Hussain, M., Ijaz, S. and Alvi, A. K. (2008) Photosynthetic performance of two mung bean (Vigna radiata) cultivars under lead and copper stress. Int. J. Agri. Biol., 10, 167-172.
  • Alscher, R.G., Erturk N. and Heath, L.S. (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot., 53, 1331-1341.
  • Baysal. O., Gursoy, Y.Z., Ornek, H., Cetinel, B. and Da Silva, J.A.T. (2007) Enhanced systemic resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato by DL-beta-aminobutyric acid under salt stress. Plant Physiol., 129, 493-506.
  • Bibi, M. and Hussain, M. (2005) Effect of copper and lead on photosynthesis and plant pigmentsin black gram (Vigna mungo L. Heaper). Bull. Environ. Contamina. Toxicol., 74, 1126-1133.
  • Blaylock, M.J., Salt, D.E., Dushenkov, S., Zakarova, O., Gussman, C., Kapulnik, Y., Ensley B.D. and Raskin, I. (1997) Enhanced accumulation of Pb2+ in Indian mustard by soil-applied chelating agents. Environ. Sci. Tech., 31, 860-865.
  • Cakmak, I. (1994) Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium and potassium-deficient leaves, but not in phosphorus-deficient leaves. J. Exp. Bot., 45, 1259-1266.
  • Cakmak, I. and Marschner, H. (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol., 98, 1222-1227.
  • Cheng, Y. L. and Song, C. P. (2006) H2O2 homeostasis and signaling in plant cells. Science in China Series C-Life Sci., 49, 1-11.
  • Di Baccio, D., Kopriva, S., Sebastiani L. and Rennenberg, H. (2005) Does glutathione metabolism have a role in the defense of poplar against zinc stress? New Phytolo., 167, 73.
  • Doke, N. (1983) Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Plant Pathol., 23, 345-357.
  • Fornazier, R. F., Forreira, R. R., Vitoria, A. P., Molina, S. M. G., Lee, P. J. and Azevedo, R. A. (2002) Effect of cadmium on antioxidant enzyme activities in sugarcane. Biol. Plant. 41, 91-97. Halliwell, B. (1987) Oxidative stress. FEBS Lett. 216, 170-171.
  • Hammerschmidt, R., Nuckles, E. M. and Kuc, J. (1982) Association of enhanced peroxidase activity with induced systemnic resistance of cucumber of Collectrotrchum lagenarium. Physiol. Mol. Plant Pathol., 20, 73-82.
  • Hegedus, A., Erdei, S. and Horvath, G. (2001) Comparative study of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci., 160, 1085-1093.
  • Hodges, D. M., De Long, J. M., Forney, C. F. and Prange, R. K. (1999) Improving the thiobarbituric acid reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interferin compounds. Planta. 207, 604-661.
  • Jacob, C., Maret, W. and Valle, B. L. (1998) Control of zinc transfer between thionein, metallothionein and zinc proteins. Proc. Nat. Acad. Sci., 95, 3489-3494.
  • Jiang, N., Luo, X., Zeng, J., Yang, Z., Zheng, L. and Wang, S. (2010) Lead toxicity induced growth and antioxidant responses in Luffa cylindrica seedlings. Int. J. Agri. Biol., 12, 205-210.
  • Jourdheuil, D., Hallen, K., Feelisch, M. and Grisham, M. B. (2000) Dynamic state of S-nitrosothiols in human plasma and whole blood. Free Rad. Biol. Med. 28, 409-417.
  • Juraci, A., De, O., Oliva, M. A., Cambraia, J. and Venegas, V. H. A. (1994). Absorption, accumulation and distribution of cadmium by two soybean cvs. Rus. Bras. Fisiol. Veg. 6, 91-95.
  • Kabata-Pendias, A. and Pendias, H. (1999) Biogeochemistry of Trace Elements (in Polish). PWN, Warszawa, Poland.
  • Lee, S.Z., Chang, L., Yang, H.H., Chen, C.M. and Liu, M.C. (1998) Absorption characteristics of lead onto soils. J. Haz. Mat., 63, 37-49.
  • Levine, M.B., Stall, A.T., Barrett, G.W. and Taylor, D.H. (1989) Heavy metal concentration during ten years of sludge treatment to an old-field community. J. Environ. Qual., 18, 411-418.
  • Malecka, A., Jarmuszkiewicz, W. and Tomaszewska, B. (2001) Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Bioch. Polon., 48, 687-698.
  • Mesquita, M.E., Carranca, C. and Menino, M. R. (2002) Influence of pH on copper-zinc competitive adsorption by a sandy soil. Environ. Toxicol., 23, 1043-1056.
  • Moloi, M.J. and Van der Westhuizen, A.J. (2006) The reactive oxygen species are involved in resistance responses of wheat to the Russian wheat aphid. J. Plant Physiol., 163, 1118-1125.
  • Morita, S., Kaminaka, H., Masumura, T. and Yanaka, K. (1999) Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress signaling. Plant Cell Physiol., 40, 417-422.
  • Noctor, G. and Foyer, C.H. (1998). Ascorbate and glutathione: keeping active oxygen under control. Ann. Rev. Plant Physiol. Plant Mol. Biol., 49, 249-279.
  • Paczkowska, M., Kozlowska, M. and Golinski, P. (2007) Oxidative stress enzyme activity in Lemna minor L. exposed to cadmium and lead. Acta Biol. Cracov., 49, 33-37.
  • Patterson, B.D. (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anals Biochem. 139, 487-492.
  • Pickering, I.J., Prince, R.C., George, M.J., Smith, R.D., George G.N. and Satt, D.E. (2000) Reduction and co-ordination of arsenic in Indian mustard. Plant Physiol., 122, 1171-1176.
  • Prakash, V., Mishra, P. K. and Mishra, M. (2009) Screening of medicinal plants extract for antioxidant activity. J. Med. Plants Res., 3, 608-612.
  • Reddy, K.J., Wang, L. and Gloss, S.P. (1995) Solubility and mobility of copper, zinc and lead in acidic environments. Plant Soil. 171, 53-58.
  • Sauve, S., McBride, M.B., Norvell, W.A. and Hendershot, W.H. (1997) Copper solubility and speciation of in situ contaminated soils: effects of copper level, pH and organic matter. Water Air Soil Poll., 100, 133-149.
  • Scandalios, J.G. (1997) Regulation and properties of plant catalases. In: Foyer C H and Mullineaux P M Causes of Photooxidative stress and Amelioration of Defense Systems in Plants 1997, CRC Press, Boca Ratan, FL. pp. 275-315.
  • Schutzendubel, A. and Polle, A. (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J. Exp. Bot., 53, 1351-1365.
  • Schutzendubel, A., Schwanz, P., Teichmann, T., Gross, K., Langenfeld-Heyser, R., Godbold, D.L. and Polle, A. (2001) Cadmium induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol., 127, 887-898.
  • Shah, K., Kumar, R.G., Verma, S. and Dubey, R.S. (2001) Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings. Plant Sci., 161, 1135-1144.
  • Sharma, P. and Dubey, R.S. (2005) Lead toxicity in plants. Braz. J. Plant Physiol., 17, 35-52.
  • Verma, S. and Dubey, R. S. (2003) Lead toxicity induces lipid peroxidation and alters the activites of antioxidant enzymes in growing rice plants. Plant Sci., 164, 645-655.
  • Yang, H., Wong, J.W.G., Yang, Z.M. and Zhou, L.X. (2001) Ability of Agrogyron elongatum to accumulate the single metal of cadmium, copper, nickel and lead and root exudation of organic acids. J. Environ. Sci., 13, 368-375.
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp