Antioxidative response of various cultivars of sorghum ( Sorghum bicolor L.) to drought stress
Автор: Singh Gurpreet, Sharma Nidhi
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 3 т.9, 2013 года.
Бесплатный доступ
The present study was conducted with the aim to identify the response of antioxidant enzyme activities in seedlings of different sorghum cultivars under mannitol stress. Seven-day old seedlings were subjected to 100-500 mM mannitol stress which resulted in the decreases in shoot/root length and relative water content thus indicating the primary response to these tissues at phenotypic level. The level of lipid peroxidation as well as the specific activity of antioxidant enzymes such as peroxidase, catalase and superoxide dismutase increased at higher conc. except at 200 mM conditions. The level of catalase and peroxidase decreased at 500 mM conc. In the two different cultivars whereas the activity of superoxide dismutase consistently increased in response to the mannitol stress. Our data demonstrate that drought responsiveness tolerance in sorghum cultivars during germination is associated with enhanced activity of antioxidant enzymes.
Catalase, drought stress, peroxidase, relative water content, superoxide dismutase, sorghum bicolor
Короткий адрес: https://sciup.org/14323760
IDR: 14323760
Список литературы Antioxidative response of various cultivars of sorghum ( Sorghum bicolor L.) to drought stress
- Aebi, H. (1984) Catalase in vitro. Methods Enzymol. 105, 121-126.
- Apel, K. and Hirt, H. (2004) Reactive oxygen species, metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55, 373-399.
- Bailly, C., Benamar, A., Corbineau, F. and Cỏme, D. (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sun flower seed as related to deterioration during accelerated aging. Physiol. Plant. 97, 104-110.
- Beauchamp, C. and Fridovich, I. (1971) Superoxide dismutase, Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276-287.
- Blokhina, O., Virolinen, E. and Fagerstedt, K.V. (2003) Antioxidants, oxidative damage and oxygen deprivation stress. Ann. Bot. 91, 179-194.
- Blum, A., Sullivan, C.Y. and Nguyen, H.T. (1997) The effect of plant size on wheat response to agents of drought stress II. Water deficit heat and ABA. Aust. J. Plant Physiol. 24, 43-48.
- Boyer, J.S. (1982) Plant productivity and environment. Science, 218, 443-448.
- Castillo, F.J. (1996) Antioxidative protection in the inducible CAM plant Sedum album L. following the imposition of severe water stress and recovery. Oecologia, 107, 469-477.
- Chaves, M.M., Maroco, J.P. and Pereira, J.S. (2003) Understanding plant responses to drought-from genes to the whole plant. Funct. Plant Biol. 30, 239-264.
- Csiszar, J., Feher-Juhasz, E., Kotai, E., Ivankovits-Kiss, O., Horvath, G.V., Mai, A., Galle, A., Tari, I., Pauk, J., Dudits, D. and Erdei, L. (2005) Effect of osmotic stress on antioxidant enzyme activities in transgenic wheat calli bearing MsALR gene. Acta Biol. Szeged. 49, 49-50.
- Dacosta, M. and Huang, B. (2007) Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in responses to drought stress. J. American Soc. Hort. Sci., 132, 319-326.
- Das, R. and Uprety, D.C. (2006) Interactive effect of moisture stress and elevated CO2 on the oxidative stress in Brassica species. J. Food Agri. Environ. 4, 298-305.
- Frensch, J. (1997) Primary response of root and leaf elongation to water deficits in the atmosphere and soil solution. J. Exp. Bot. 48, 985-999.
- Gunes, A., Pilbeam, D., Ina, A. and Coban, S. (2008) Influence of silicon on sunflower cultivars under drought stress, I, Growth, antioxidant mechanisms and lipid peroxidation. Commun. Soil Sci. Plant Nut. 39, 1885-1903.
- Harris, H. and Hopkinson, D.A. (1976) Handbook of Enzyme Electrophoresis in Human Genetics, North-Holland, Amsterdam.
- Heath, R.L. and Packer, L. (1968) Photoperoxidation in isolated chloroplasts, Kinetics and Stoichiometry of Fatty Acid Peroxidation. Arch. Biochem. Biop. 125, 189-198.
- Horváth, E., Pál, M., Szalai, G., Páldi, E. and Janda, T. (2007) Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants. Biol. Plant. 51, 480-487.
- Klapheck, S., Zimmer, I. and Cosse, H. (1990) Scavenging of hydrgen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol. 31, 1005-1013
- Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
- Liang, Z., Yang, J., Shao, H. and Han, R. (2006) Investigation on water consumption characteristics and water use efficiency of poplar under soil water deficits on the Loess Plateau. Colloids and Surfaces B, Biointerfaces, 53, 23-28.
- Lichtenthaler, H.K. (1996). Vegetation stress, An introduction to the stress concept in plants. J. Plant Physiol. 148, 4-14.
- Liu, E. H. (1973) A simple method for determining the relative activities of individual peroxidase isoenzymes in a tissue extract. Anal. Biochem. 56, 149-154.
- Manivannan, P., Abdul Jaleel, C., Kishorekumar, A., Sankar, B., Somasundaram, R., Sridharan, R. and Panneerselvam, R. (2007) Changes in antioxidant metabolism of Vigna unguiculata (L.) Walp. by propiconazole under water deficit stress. Colloids and Surfaces-Biointerfaces, 57, 69-74.
- Marron, N., Delay, D., Petit, J.M., Dreyer, E., Kahlem, G., Delmotte, F.M. and Brignolas, F. (2002) Physiological traits of two Populus × euramericana clones, Luisa Avanzo and Dorskamp, during a water stress and re-watering cycle. Tree Physiol. 22, 849-858.
- McKersie, B.D. and Leshem, Y.Y. (1994) Stress and stress coping in cultivated plants. Kluwer Academic Publishes, London.
- Moller, I.M., Jensen, P.E. and Hansson, A. (2007) Oxidative modifications to cellular components in plants. Annu. Rev. Plant Biol. 58, 459-481.
- Munns, R. (2002) Comparative physiology of salt and water stress. Plant Cell Environ. 25, 239-250.
- Munns, R., Passioura, J.B., Guo, J., Ehazen, O., and Cramer, G.R. (2000) Water relations and leaf expansion, Importance of timing. J. Exp. Bot. 51, 1495-1504.
- Nayyar, H. and Gupta, D. (2006) Differential sensitivity of C3 and C4 plants to water deficit stress, Association with oxidative stress and antioxidants. Environ. Exp. Bot. 58, 106-113.
- Osorio, J., Osorio, M.L., Chaves, M.M. and Pereira, J.S. (1998) Water deficits are more important in delaying growth than in changing patterns of carbon allocation in Eucalyptus globulus. Tree Physiol. 18, 363-373.
- Passioura, J.B. (1988) Root signals control leaf expansion in wheat seedlings grown in drying soil. Aust.J.Plant Physiol. 15, 687-693.
- Pastori, G.M. and Foyer, C.H. (2002) Common components, networks, and pathways of cross-tolerance to stress. The central role of ‘redox’and abscisic acid-mediated controls. Plant Physiol. 129, 7460-7468.
- Queiroz, C.G.S., Alonso, A., Mares-Guia, M. and Magalhaes, A.C. (1998) Chilling-induced changes in membrane fluidity and antioxidant enzyme activities in Coffea arabica L. roots. Biol. Plants, 41, 403-413.
- Reddy, A.R., Chaitanya, K.V., Jutur, P.P. and Sumithra, K. (2004) Differential antioxidative responses to water stress among five mulberry (Morus alba L.) cultivars. Environ. Exp. Bot. 52, 33-42.
- Roth, E.F. and Gilbert, H.S. (1984) Pyrogallol assay for SOD, absence of a glutathione artifact. Anal. Biochem. 137, 50-53.
- Sharma, P. and Dubey, R.S. (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzyme in growing rice seedling. Plant Growth Regul. 46, 209-221.
- Sofo, A.B., Dichio, C., Xiloyannis, C. and Masia, C. (2004). Lipoxygenase activity and proline accumulationin leaves and roots of olive trees in response to drought stress. Physiol. Plant. 121, 58-65.
- Spollen, W.G., Sharp, R.E., Saab, I.N. and Wu, Y. (1993) Regulation of cell expansion in roots and shoots at lowwater potentials. In, Water Deficits, Plant Responses from Cell to Community. Smith J. A. C., Griffiths H., Eds. BIOS Scientific Publishers, Oxford, pp. 37-52.
- Tas, S. and Tas, B. (2007) Some physiological responses of drought stress in wheat genotypes with different ploidity in Turkiye. World J.Agri. Sci. 3, 178-183.
- Taulavuori, E., Hellstrom, E., Taulavuori, K. and Laine, K. (2001) Comparison of two methods used to analyse lipid peroxidation from Vaccinium myrtillus during snow removal, reacclimation and cold acclimation. J. Exp.Bot. 52, 2375-2380.
- Tsugane, K., Kobayashi, K., Niwa, Y., Ohba, Y., Wada, K. and Kobayashi, H. (1999) A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. Plant Cell, 11, 1195-1206.
- Xiao, X., Xu, X. and Yang, F. (2008) Adaptive responses to progressive drought stress in two Populus cathayana populations. Silva Fennica, 42, 705-719.
- Xu, P.L., Guo, Y.K., Bai, J.G., Shang, L. and Wang, X.J. (2008) Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiologia Plantarum, 132, 467-478.