Apoptosis of testicular germinal tissue in condition disorders of the nervous and epiphyseal regylations

Expression of enzymes involved in apoptosis and DNA repair in spermatogenic cells – procaspase-3 and PARP-1 (p116/25), was detected in male of white rats with epiphyseal deficiency and impaired peripheral innervation of the testes. Determination of enzyme was carried out in the dark (1 hr) and light (13 h) time for two days. Epiphyseal deficiency caused a significant increase in the expression of the researched enzymes in maturing germ cells and the disappearance of their circadian rhythm. Peripheral denervation of the testes, without leading to the disappearance of the daily rhythm of enzymes expression caused a significant increase the level of procaspase-3 and PARP-1. These findings indicate the increase a damage in the DNA structure of the spermatogenic cells at interruption of some steps of outside the hypotalamic regulation.