Ascorbic acid and α-tocopherol minimize salt-induced wheat leaf senescence

Ascorbic acid and α-tocopherol minimize salt-induced wheat leaf senescence

Автор: Farouk S

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 3 т.7, 2011 года.

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Background Leaf senescence is an oxidative process, and most of the catabolic events involved in senescence are propagated irreversibly once initiated. Results Salinity hastened the senescence of wheat flag leaves, decreased the concentrations of chlorophyll, total carotenoids, ascorbic acid, total phenol, calcium, potassium, magnesium, K+/Na+ ratio and soluble proteins, as well as the activities of catalase and peroxidase. Conversely, salinity increased sodium, chloride, and the chlorophylla:b ratio, as well as membrane permeability, hydrogen peroxide, and malondialdehyde synthesis. Both antioxidants application reduced the hydrogen peroxide accumulation, lipid peroxidation, membrane permeability, sodium and chloride content over control plants. The antioxidants enzyme activities were significantly increased by antioxidant spray. Enhanced accumulation of ascorbate, phenol, carotenoids, calcium, potassium and magnesium was seen in antioxidants-sprayed plants compared with control plants at 65 days after sowing. Under moderate and sever salinity levels application of both antioxidants alleviated the harmful effects of salinity on leaf senescence related parameter. The higher levels of antioxidants and low level of H2O2 in flag leaf may be the prerequisite for delayed leaf senescence in antioxidants-sprayed plants. Conclusions It can be concluded that ascorbic acid-sprayed plants can postpone the leaf senescence by peroxide/phenolic/ascorbate system which is involved in scavenging the ROS produced during leaf senescence.

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Antioxidants, malondialdehyde, salinity, senescence, wheat

Короткий адрес: https://sciup.org/14323539

IDR: 14323539

Список литературы Ascorbic acid and α-tocopherol minimize salt-induced wheat leaf senescence

  • Abd El-Aziz, Nahed, G., Mozher, Azza, A.M. and El-Habba, E. (2006) Effect of foliar spraying with ascorbic acid on growth and chemical constituents of Khaya senegalensis grown under salt condition. Amer-Eurasian J. Agric and Environ. Sci., 1(3), 207 -217.
  • Alam, S.M. (1994) Nutrient uptake by plants under stress conditions. In, Passarakli, M. (ed.), Handbook of plant and crop stress, Marcel Dekker, New York. pp. 227-246.
  • Alia, P., Saradhi, P. and Mohanty, P., (1993) Proline in relation to free radical production in seedlings of Brassica juncea raised under sodium chloride stress. Plant and Soil, 156, 497-500.
  • Athar, H.R., Khan, A. and Ashraf, M. (2008) Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environ. Exp. Botany, 63, 224 -231.
  • Azooz, M.M. (2004) Proteins, sugars and ion leakage as a selection criterion for the salt tolerance of three sorghum cultivars at seedling stage grow under NaCl and nicotinamide. Int. J. Agri. Biol., 6(1), 27 -35.
  • Bassuony, F.M., Hassanein, R.A., Baraka, D.M. and Khalil, R.R. (2008) Physiological effects of Nictinamide and Ascorbic acid on Zea mays plant grown under salinity stress. II changes in nitrogen constituents, protein profiles, protease enzymes and certain inorganic cations. Austrailan J. Basic and applied science, 2(3), 350 -359.
  • Bjцrkman, O. (1981) Responses to different quantum flux densities. In, Lange, O.L., Nobel, P.S., Osmond, C.B. and Ziegler, H. (eds), Physiological Plant Ecology, Spring-Verlag, Berlin. pp. 57-107.
  • Bowler, C., Montague, M.V. and Inze, D. (1992) Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol., 43, 83 -116.
  • Bradford, M.M. (1976) A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248 -254.
  • Breusegem, F.V. and Dat, J.F. (2006) Reactive oxygen species in plant cell death. Plant physiol., 141, 384-390.
  • Burton, G.W., Doba, T., Gabe, E.J., Hughes, L., Lee, F.L., Prasad, L. and Ingold, K.U. (1985) Autoxidation of biological molecules. 4. Maximizing the antioxidant activity of phenols. J. Am. Chem. Soc., 107, 7053 -7065.
  • Conklin, P.L. and Barth, C. (2004) Ascorbic acid, a familiar small molecule intertwined in the response of plants to ozone, pathogens, and the onset of senescence. Plant Cell Env., 27, 959 -971.
  • Davey, M.W., Mantagu, M.V., Dirk, I., Maite, S., Angelos, K., Smirnoff, N., Binenzie, I.J.J., Strain, J.J., Favell, D. and Fletcher, J. (2000) Plant ascorbic: acid chemistry, function, metabolism, bioavailability and effects of processing. J. Sci. Food and Agri., 80, 825 -850.
  • Djanaguiraman, M., Sheeba, J.A., Devi, D.D. and Bangarusamy, U. (2005) Response of cotton to Atonik and TIBA for growth, enzymes and yield. J. Biol. Sci., 5, 158 -162.
  • El-Bassiony, H.M. (2005) Physiological responses of wheat to salinity alleviation by nicotinamide and tryptophan. Int. J. Agric. Biol., 653 -659.
  • Fang, Z., Bouwkamp, J. and Solomos, T. (1998) Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L. J. Exp. Bot., 49, 503 -510.
  • Flowers, T.J. and Dalmond, D. (1992) Protein synthesis in halophytes: the influence of potassium, sodium and magnesium in vitro. Plant Soil, 146, 153 -161.
  • Foyer, C.H. and Harbinson, J. (1994) Oxygen metabolism and the regulation of photosynthetic electron transport. IN, Foyer, C.H. and Mullineaux, P.M. (eds), Cause of photooxidative stress and amelioration of defence system in plants. CRC Press, Boca Raton, FL. pp. 1-42.
  • Foyer, C.H., Lelandais, M. and Kunert K.J. (1994) Photoxidative stress in plants. Physiol. Plant., 92, 696 -717.
  • Goncalves, J.F., Becker, A.G., Crgnelutti, D., Tabaldi, L.A., Pereira, L.B., Battisti, V., Spanevello, R.M., Morsch, V.M., Nicoloso, F.T. and Schetinger M.R.C. (2007) Cadmium toxicity causes oxidative stress and induces response of the antioxidant system in cucumber seedlings. Braz. J. Plant Physiol., 13(3), 223 -232.
  • Halliwell, B., Asechbach, R.I., Loliger, J. and Aruoma, O.I. (1995) The characterization of antioxidants. J. Food Chem. Toxicol., 33, 601 -617.
  • Hameed, A., Naseer, S., Iqbal, T., Syed, H. and Ahsanulhaq, M. (2008) Effects of NaCl salinity on seedlings growth, senescence, catalase and protease activities in two wheat genotypes differing in salt tolerance. Pak. J. Bot., 40(3), 1043-1051.
  • Hanson, E.M. and Munns, D.N. (1988) Effect of CaSo4 and NaCl on mineral content of Leucaena leucocephata. Plant Soil, 107, 101-105.
  • Hung, S.H., Yu, C.W. and Lin, C.H. (2005) Hydrogen peroxide functions as a stress signal in plants. Bot. Bull. Acad. Sin., 46, 1 -10.
  • Hussein, M.M., Balbaa, L.K. and Gaballah, M.S. (2007) Developing a salt tolerant cowpea using alpha tocopherol. J. Applied Sci. Res., 3(10), 1234 -1239.
  • Jaleel, C.A., Manivannan, P. and Lakshmanan, G.M.A. (2007) NaCl as a physiological modulator of proline metabolism and antioxidant Phyllanthus amarus. C R Biologies, 330, 806 -813.
  • Jang, H.D., Chang, K.S., Huang, Y.S., HSU, C.I., Lee, S.H. and Su, M.S. (2007) Principal phenolic phytochemical and antioxidant activities of three Chinese medicinal plants. Food Chem., 103, 749 -756.
  • Krieger-Liszkay, A. and Trebst, A. (2006) Tocopherol is the scavenger of singlet oxygen produced by the triplet states of chlorophyll in the PSII reaction centre. J. Exp. Bot., 57(8), 1677 -1684.
  • Kukavica, B. and Jovanovic, S.V. (2004) Senescence-related changes in the antioxidant status of ginkgo and birch leaves during autumn yellowing. Physiol. Plant., 122, 321 -327.
  • Leidi, E.O., Nogales, R. and Lips, S.H. (1991) Effect of salinity on cotton plants grown under nitrate or ammonium nutrition at different calcium levels. Field Crops Res., 26, 35 -44.
  • Lichtenthaler, H.K. and Wellbum, A.R. (1985) Determination of total carotenoids and chlorophylls A and B of leaf in Different Solvents. Biol. Soc. Trans., 11, 591 -592.
  • Loggini, B., Scartazza, A., Brugnoli, E. and Navari-Izzo, F. (1999) Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiol., 119, 1091 -1099.
  • Lutts, S., Kinet, J.M. and Bouharmont, J. (1996) NaCL-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann. Bot., 78, 389 -398.
  • Mayer, J.M., Hrovat, D.A., Thomas, J.L. and Bordon, W.T. (2002) Protein-coupled electron transfer vs. hydrogen atom transfer in enzl/tolune, methoxyl/methanol, and phenoxyl/phenol self exchange reactions. J. Am. Chem. Soc., 124, 11142 -11147.
  • Metile, P., Hortensteiner, S. And Thomas, H. (1999) Chlorophyll degradation. Ann. Rev. Plant Physiol. Plant Mol. Biol., 50, 67-95.
  • Mittler, R. (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci., 7, 405 -410.
  • Mшller, I.M., Jensen, P.E. and Hansson, A. (2007) Oxidative modifications to cellular components in plants. Annu. Rev. Plant Biol., 58, 459 -481.
  • Munnґe-Bosch, S. (2007) Aging in perennials. Crit. Rev. Plant Sci., 26, 123 -138.
  • Navabpour, S., Morris, K., Allen, R., Harrison, E., Mackerness, S.A.H. and Buchanan-Wollaston, V. (2003) Expression of senescence enhanced genes in response to oxidative stress. J. Exp. Bot., 54, 2285 -2292.
  • Noctor, G. and Foyer, C.H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol., 49, 249 -279.
  • Norman, G.R. and Streiner, D.L. (2003) PDQ Statistics, 3rd Ed. BC Deckker Inc, London.
  • Pavet, V., Ollimos, E., Kiddle, G., Kumar, S., Antonaiw, J., Alvarez, M.E. and Foyer, C.H. (2005) Ascorbic acid deficiency activates cell death and disease resistance in Arabidopsis thaliana. Plant Physiol., 139, 1291 -1303.
  • Prochazkova, D., Sairam, R.K., Srivastava, G.C. and Singh, D.V. (2001) Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Science, 161, 765 -771.
  • Rao, M.V., Paliyath, G., Ormrod, D.P., Murr, D.P. and Watkins, C.B. (1997) Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2 metabolizing enzymes. Salicylic acid-mediated oxidative damage requires H2O2. Plant Physiol., 115, 137 -149.
  • Reuveni, M and Reuveni, R. (1995) Efficacy of foliar application of phosphate in controlling powdery mildew fungus on field grown wine grapes: effects on cluster yield and peroxidase activity in berries. Journal of Phytopathology, 143, 21 -25.
  • Richard, L.A. (1954) Diagnosis and Improvement of saline and alkali soils.U.S. Dept. of Agric. Handbook No 60.
  • Sadasivam, S. and Manickam, A. (1996) Biochemical methods, Second edition, New age international. India.
  • Sairam, R.K. and Srivastava, G.C. (2000) Induction of oxidative stress and antioxidant activity by hydrogen peroxide treatment in tolerant and susceptible wheat genotypes. Biol. Plant., 43, 381 -386.
  • Seckin, B., Sekmen, A.H. and Tьrkan, I. (2009) An enhancing effect of exogenous mannitol on the antioxidant enzyme activities in roots of wheat under salt stress. J. Plant Growth Regul., 28, 12 -20.
  • Shalata, A. and Neumann, P.M. (2001). Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. J. Exp. Bot., 52(364), 2207 -2211.
  • Shao, H.B., Liang, Z.S., Shao, M.A. and Wang, B.C. (2005) Changes of some physiological and biochemical indices for soil water deficits among 10 wheat genotypes at seedlings stage. Colloids and Surfaces B: Biointerfaces, 42(2), 107 -113.
  • Singleton, V.L. and Rossi, J.A. (1965) Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Amer. J. Enol. Vitic., 16, 144 -158.
  • Sylvester-Bradley, R., Scott, R.K. and Wright, C.E. (1990) Physiology in the production and improvement of cereals. Home-Grown Cereals Authority Research Review, vol. 18. HGCA, London.
  • Teranishi, Y., Tanaka, A., Osumi, M. And Fukui, S. (1974) Catalase activity of hydrocarbon utilizing candida yeast. Agr. Biol. Chem., 38, 1213 -1216.
  • Thomas, H., Ougham, H., Canter, P. and Donnison, I. (2002) What stay-green mutants tell us about nitrogen remobilization in leaf senescence. J. Exp.Bot., 53, 801-808.
  • Vieira Santos, C.L., Campos, A., Azevedo, H. and Caldeira, G. (2001) In situ and in vitro senescence induced by KCL stress: nutritional imbalance, lipid peroxidation and antioxidant metabolism. J. Exp. Bot., 52 (355), 351 -360.
  • Wise, R.R. and Naylor, A.W. (1987) Chilling enhanced photo-oxidation. Plant Physiology, 83, 278 -282.
  • Zabalza, A., Gaston, S., Sandalio, L.M., Del Rio, L.A. and Royuela, M. (2007) Oxidative stress is not related to the mode of action of herbicides that inhibit acetolactate synthase. Environ. Exp. Bot., 59, 150 -159.
  • Zhao, H., Dai, T., Jing, Q., Jiang, D. and Cao, W. (2007) Leaf senescence and gain filling affected by post-anthesis high temperature in two different wheat cultivars. Plant Growth Regul., 51, 149 -158.
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