Polyacrylamide biodegradation by soil microflora and bacteria containing amidase

The effect of long-term incubation of samples sod-meadow heavy loam and alluvial meadow soils, and oil-saturated sandstone with polyacrylamide (PAA) on the number of amidase containing bacteria in the soil and the total amidase activity of soil extract was studied. It was determined that in the alluvial and loamy soil during prolonged incubation with PAA content of microorganisms having amidase activity was increased. Amidase activity of soil extracts for the period from 6 to 34 weeks was increased by several orders of magnitude. After 38 weeks in the alluvial soil decline of PAA was marked, that achieved 10-37% of the introduced quantity of water-retaining agent. It was shown that Alcaligenes faecalis 2, and Acinetobacter guillouiae 11h on liquid mineral media with carbon source and the PAA as a source of nitrogen formed biofilms on the hydrogel beads.