Blood biochemical parameters in reservoir hosts under ixodid tick-borne borreliosis

Currently, there is a wide distribution of natural foci of ixodid tick-borne borreliosis (ITB, Lyme disease) in Europe, Asia, Australia and America, as well as high infection rate of people and animals. Studies have shown that this new natural focal infection occupies a leading position in terms of morbidity and socio-economic damage. Many pathogenic microorganisms, including Borrelia burgdorferi , which cause disease in humans and animals, persist in certain natural foci. Ticks feed on different animals at different stages of development. The size of the tick population mainly depends on the number of adult reservoir hosts. The research, for the first time, revealed in ITB infection reservoir hosts with diagnostically significant titers of antibodies to В. burgdorferi the blood serum biochemical parameters which indicate the pathological effect of the pathogen on the body of wild animals, the development of multiple organ failure and harm to their health. The aim was to study the natural foci of ixodid tick-borne borreliosis and to obtain new data on the epizootology of the disease, including an assessment of the effect of parasitism of ixodid ticks infected with B. burgdorferi on the blood chemical composition of reservoir hosts, the mountain hare and moose. Blood serum from adult males of mountain hare ( n = 11) was used, including 5 samples that had diagnostically significant titers of antibodies to B. burgdorfer i in the indirect immunofluorescence reaction (IRIF) (1:40 and 1:80), and 6 samples that did not have diagnostically significant titers of antibodies to B. burgdorferi (control). We also studied blood serum from moose ( n = 114) of different sex and age groups (animals aged 6-7 months and adults), including 24 samples with diagnostically significant titers of antibodies to B. burgdorferi and 90 samples from clinically healthy animals (Kirov region), including individuals whose sera in the NRIF did not have diagnostically significant titers of antibodies to B. burgdorferi . Animals were hunted during scientific shooting during the autumn hunting seasons of 2005-2020. Blood samples for laboratory studies were taken from the jugular vein immediately after the animal was shot. Anti-borreliosis antibodies in blood serum was detected in NRIF test using B. afzelii corpuscular antigen (strain Ip-21) and fluorescein isothiocyanate (FITC) labeled luminescent immune serum against globulins of various animal species (rabbit, dog, bull, pig, chicken). Biochemical studies of blood serum were performed using a semi-automatic analyzer Biochem SA (High Technology Inc., USA) with a set of reagents (Eco-Service, Russia) to measure the concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, lactate dehydrogenase (LDH), alpha-amylase, total protein, albumin, total bilirubin, direct bilirubin, creatinine and cholesterol. It was found that the animals with diagnostically significant titers of antibodies to B. burgdorferi had statistically significant (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }