Biomarkers for modeling of cancer-specific tumor-associated macrophages ex vivo

Biomarkers for modeling of cancer-specific tumor-associated macrophages ex vivo

Автор: Sudarskikh T.S., Larionova I.V., Rakina M.A., Kzhyshkowska J.G.

Журнал: Сибирский онкологический журнал @siboncoj

Рубрика: Лабораторные и экспериментальные исследования

Статья в выпуске: 4 т.23, 2024 года.

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Introduction. Tumor-associated macrophages (TAMs) are essential innate immune cells in the tumor microenvironment. TAMs can stimulate cancer cell proliferation and primary tumor growth, angiogenesis, lymphangiogenesis, cancer cell invasiveness in vessels and metastatic niche formation as well as support chemotherapy resistance. TAMs are phenotypically diverse both in various cancer localizations and in intratumoral heterogeneous compartments. Tumor-specific modeling of TAMs is necessary to understand the fundamental mechanism of pro- and anti-tumor activity, to test their interaction with existing therapies, and to develop TAM- targeted immunotherapy. Aim of study: To investigate cancer-specific transcriptomic features of ex vivo human TAM models. Material and Methods. Here we compared transcriptomic profiles of TAMs for breast, colorectal, ovarian, lung, and prostate cancers ex vivo . Human monocytes were isolated from buffy coats, and then stimulated by the tumor cell conditioned medium ex vivo . Using real-time PCR, we quantified the expression of key TAM biomarkers including inflammatory cytokines, scavenger-receptors, angiogenesis-regulating genes, and matrix remodeling factors.

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Tumor-associated macrophages, m1/m2 classification, cytokines, scavenger-receptors

Короткий адрес: https://sciup.org/140307090

IDR: 140307090   |   DOI: 10.21294/1814-4861-2024-23-4-54-65

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