Biofilms of hydrocarbon-oxidizing bacteria from anthropogenic soils in Kogalym

The article is devoted to the determination of the biological properties and conditions of biofilm formation by hydrocarbon-oxidizing bacteria for their further use in oil pollution treatment and remediation of anthropogenic disturbed soils. The object of the study is hydrocarbon-oxidizing microorganisms isolated from soil samples from Kogalym: Bacillus alcalophilus WS-3027B, B. funiculus LY-2403G, B. halodurans MH-3011N, B. niacini TC-8101S, B. psychrodurans LV-1106E, Curtobacterium flaccumfaciens AE-0851V. Biofilm formation activities were evaluated by the crystal violet staining method for assessing biofilm formation degree in the stationary phase according to O'Toole et al. Among the hydrocarbon-oxidizing microorganisms, B. alcalophilus WS-3027B and C. flaccumfaciens AE-0851V were found to form dense biofilms, while B. halodurans MH-3011N, B. niacini TC-8101S, B. funiculus LY-2403G, and B. psychrodurans LV-1106E exhibited moderate biofilm production. Optimal conditions for the formation of biofilms by the studied microorganisms have been established. The conducted study of biofilm formation of hydrocarbon-oxidizing bacteria opens up prospects for the use of these strains as active destructors of petroleum products in the bioremediation of contaminated soils.