Biosynthesis of transmembrane photo-transforming protein [2н] bacteriorhodopsin, labelled with deuterium on residues of aromatic amino acids [2,3,4,5,6- 2H 5] Phe, [3,5- 2H 2] Tyr и [2,4,5,6,7- 2H 5] Trp

The microbial micropreparative synthesis of transmembrane photo-transforming protein bacteriorhodopsin (output 8-10 mg), labeled with deuterium on residues of [2,3,4,5,6- 2H 5]phenylalanine, [3,5- 2H 2]tyrosine, and [2,4,5,6,7- 2H 5]tryptophan was carried out that is important for nanoindustry of new modern domestic photo-transforming nanomaterials and molecular bioelectronics. The protein was allocated from membranes of photoorganoheterotrophic halobacterium Halobacterium halobium by cellular lysis by distilled water, processing of bacterial biomass by ultrasound, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, with the subsequent solubilization of final product with 0.5 % SDS-Na and fractionation by methanol. The homogenity of the synthesized product, and the selectivity of deuterium encorporation into the molecule was proved by combination of preparative and analytical protein methods including elecrtophoresis in 12.5 % PAAG with 0.1 % SDS-Na, gel filtration chromatography on Sephadex G-200, reverse-phase HCLP, electron impact mass-spectrometry of methyl esters of N-Dns-derivatives of amino acids.