Biostimulating influence of synthetic humic-like substances on Cucumis sativus L. earle stage growth according to IR Fourier transform spectroscopy data

The research objective was IK-Fourier spectroscopic research of the dynamics of the processes of the formation of primary metabolites in root system of the sprouts of test plants of Cucumis Sativus L., occurring at their growing in the solutions of synthetic humic-like substances. The problems of synthesis of humic-like substances on the basis of D-glucose and p-aminobenzoic acid, carrying out vegetative experiments on test plants (Cucumis Sativus L were solved.) with additives of the synthesized products and IK-Fourier's analysis of the ranges of sprouts by means of reference of the main strips characterizing the change of the maintenance of primary metabolites in root system of plants. In a series of laboratory experiments, it was found that aqueous solutions of synthesized products have noticeable biostimulating effect on the cucumber (Cucumis Sativus L.) seeds growth at low content of active substance (0.004-0.002 %), increasing with the concentration decrease. For 0.008 % solution, inhibition of growth processes was noted in comparison with control. Structural changes in the root system of the test plant seedlings occurred during their germination in the medium of solutions of synthesized substances were investigated using IR- Fourier transform spectroscopy method. The spectrum of the sample germinated in 0.008 % solution shows a band profile practically similar to the spectrum of the control sample both in terms of the position of the signals and their intensity, which for a number of IR bands is even lower than the control. The spectra of the samples germinated in 0.004 and 0.002 % solutions show an increase in band intensity at 1240 cm-1 and especially in the 960-1200 cm-1 region. The increase in the intensity of IR bands is associated with the formation of appropriate substances, in particular, the biosynthesis processes of organophosphorus compounds and also with stimulation the carbohydrates accumulation. The investigated products were synthesized from available and environmentally safe biologically active substances, which, together with their growth-activating action and simple preparation, made it promising to further study the activity mechanisms of these compounds on plant systems in order to create the production technology of effective preparations for plant industry on its basis.