Changes in fatty acid composition in leaf lipids of canola biotech plants under short-time heat stress
Автор: Sakhno Liudmyla O., Slyvets Mariia S., Korol Nataliia A., Karbovska Nataliia V., Ostapchuk Andriy M., Sheludko Yuriy V., Kuchuk Mycola V.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.10, 2014 года.
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In order to study the influence of expression of heterologous genes of different origin ( cyp 11A1 and des C) on canola thermotolerance improvement on leaf membrane level the fatty acid composition was analyzed under short-time heat test. C yp 11A1 gene encodes cytochrome P450 SCC from bovine adrenal cortex mitochondria and was shown to affect the biosynthesis of steroid compounds. DesC gene encodes ∆9-acyl-lipid desaturase of cyanobacterium Synechococcus vulcanus. Decrease in palmitlinolenic acid content and index unsaturation as well as increase in total fatty acid and palmitic acid content were identified in cyp 11A1 canola in comparison with wild-type plants in stressfull conditions. But control and des C plants demonstrated similar changes in saturated (16:0), trienoic (16:3 and 18:3) fatty acid quantity, total fatty acid content and index unsaturation. Heterologous des C gene expression did not influence fatty acid composition and did not give advantages for plant under heat. Integration of cyp 11A1 gene in canola led to thermotolerance improvement on membrane level.
Brassica napus, cyp11a1, desc, fatty acids, heat stress
Короткий адрес: https://sciup.org/14323863
IDR: 14323863
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