The new bisphosphonate on the basis of etidronate ions of lanthanides and calcium to restore bone defects in animals in the experiment

As an experimental model were used 36 rabbits aged 6 - 10 months with body masses 2500-2800g, which were injured by perforating a cortical plate in the proximal section of the tibia. On the 3rd and 5th days in the test group of animals a preparation of a lanthanide and calcium ions in a dose of 0.2ml were twice injected into the formed defects. Morphological analysis of the bone defect edges and tissue, filling the perforated hole, was performed on the 7th, 14th, 28th and 56th days. For the local change control on the injury zone researches were conducted on the computer tomography Brilliance 64 (Philips). A cut processing of reparative zone was conducted in the multiplanaris reconstruction mode in sagittalis plate. The investigated drug has improved the healing process of the bone defect already on the 7th day of the experiment that was characterized by a smaller area of the perforated hole and a smaller amount of leukocyte-necrotic masses in the hole. By the 14th day the reparative regeneration parameters were also significantly different from the control ones: the area of granulation tissue was reducing simultaneously transforming into the connective tissue that led to the defect closing with nonlamellar bone by the 28th day. At the later stages in the area of injury a formed lamellar bone was visualized. The average value of the density in the first 10 days after surgery in rabbits of the test group (where the drug was injected into the experimentally made bone defect) is 1.5-2 times higher than that in the rabbits of the comparison group. Further terms of the experiment were characterized by a continuing trend towards increasing the density of regenerate in both groups.