Detection of the genes coding ectoine biosynthesis enzymes for halotolerant actinobacteria isolated from the soil of area of industrial development of the Upperkamian salted basin

Two primer systems were designed, polymerase chain reaction conditions and PCR mixture composition for amplification of the ect-operon fragment comprising ectB and ectC-genes, determining L-2,4-diaminobutyrate transferase and ectoine synthase, on the DNA template of the bacteria of the genera Rhodococcus, Microbacterium and Brevibacterium were developed. Screening of 9 bacterial strains isolated from the soil of the Upperkamian salted basin industrial development area using the primer systems was carried out. A specific product amplification of target genes was obtained on a DNA template of strains of Brevibacterium sp. B-R and Microbacterium sp. Y6, as well as representatives of phylogenetic groups of R. erythropolis and R. rhodochrous of the genus Rhodococcus. On the genomic DNA of the strain Microbacterium sp. SMB33 specific product was not synthesized, which may indicate a difference in the genetic determinants of the ectoine synthesis of this strain from known ones.