Differential diagnosis of severe acute pancreatitis and lightning-fast pancreatitis by atomic force microscopy

Introduction. Severe acute pancreatitis is a particularly dangerous form of acute pancreatitis, which is highly likely to turn into pancreatic necrosis, which requires early diagnosis of this condition with the immediate use of all necessary medical and surgical measures.The aim of the work is to correlate the data of atomic force microscopy of the erythrocyte membrane and the severity of acute pancreatitis to predict and early detect the severity of the course.Materials and methods. 2 groups of patients with acute pancreatitis with a disease duration of three days from the onset of the disease to the moment of hospitalization were identified. As part of the ongoing study, the condition of peripheral blood erythrocytes of patients was assessed and compared with erythrocytes of healthy people by atomic electron microscopy. The evaluation of the obtained data was carried out by statistical processing.Results. The surface membrane of erythrocytes was scanned using atomic force microscopy. When receiving images, it was revealed that, unlike the erythrocytes of healthy people, erosions are noted on the erythrocyte membrane in severe pancreatitis. With lightning-fast pancreatitis, the number of erosions increases significantly, the area and depth also becomes larger.Conclusion. The introduction of atomic force microscopy of peripheral blood erythrocytes into clinical practice can provide significant assistance in the differential diagnosis of severe and lightning-fast acute pancreatitis. Timely and correct determination of the severity of acute pancreatitis will allow to change the tactics of treatment of patients and improve both immediate and long-term results.