Dynamics of laser speckle imaging of blood flow and morphological changes in tissues with a full time local ischemia of pancreas

Автор: Alexandrov D.A., Timoshina P.A., Tuchin V., Maslyakova G.N., Palatova T.V., Skorokhod A.A., Lukashevich K.A., Yarovoy A.S.

Журнал: Саратовский научно-медицинский журнал @ssmj

Рубрика: Макро- и микроморфология

Статья в выпуске: 4 т.10, 2014 года.

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The purpose: to establish influence of a full ischemia of different duration and the subsequent reperfusionon pathology development in pancreas of rats by means of laser speckle-visualization and lifetime digital microscopy. Materials and Methods. The work has been performed on 42 white rats of line Wistar in weight of 200-250 Research of properties of a blood-groove was made by means of methods laser Doppler flowmetry, digital biomicroscopy and a method of laser speckle-contrast visualization. Results. After the termination of a 5-minute full ischemia the speed of bloodflow has been increased in 2-3 times, clinic pancreatic necrosis is marked does not develop. After the termination of 20-minute full ischemia the increase in speed of a bloodflow did not occur, there were morphological and clinical signs of pancreatic necrosis. Conclusion, the efficiency of monitoring of microhemodynamics of pancreas in rats by the method of speckle-capillary of full field has been shown. Multidirectional phase of perfusion changes in pancreas have been revealed after reversible infringement of blood supply of different duration.

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Pancreas, reperfusion, speckle imaging

Короткий адрес: https://sciup.org/14918014

IDR: 14918014

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