Design of oligonucleotide system and optimization of conditions for amplification of ECF-genes of bacteria of genus Salinicola of Halomonadaceae family

Search for nucleotide sequences of ec/-operon from bacteria of Halomonadaceae family was carried out in open database of National Center for Biotechnology Information (USA) (https://www.ncbi.nlm.nih.gov). Analysis of aligned 33 nucleotide sequences from the members of genera Salinicola, Halomonas, Chromohalobacter, Kushneria, Cobetia and Halotalea allowed revealing conserved regions. The system of oligonucleotide primers was designed to these in order to amplify the ec/-operon fragment including genes: ec/A, which encodes L-2,4-diarninobutyrate acetyltransferase and eciB, which determines L-2,4-diaminobutyrate aminotransferase. Conditions for polymerase chain reaction were experimentally selected, as well as PCR-rnixture composition to amplify the ec/-operon site on DNA of bacteria of genus Salinicola.