Effect of 24-epibrassinolide on lipid peroxidation and proline in three Brassica species under temperature stress

Abiotic stresses, such as temperature, drought and salinity are serious threats to agriculture. Temperature stress is the primary cause of crop loss worldwide, reducing average yields for most major crop plants. In present study role of 24-epibrassinolide in three Brassica species ( B. carinata, B. juncea and B. napus ) on lipid peroxidation and proline under temperature stress was investigated. Seeds were given temperature treatments (4, 14, 24, 34 and 44°C) for 5 hours alone or in combination with EBR (10 -11, 10 -9 and 10 -7 M). Temperature stress whether low and high causes stress in terms of lipid peroxidation. High temperature causes more stress as compared with low temperature stress, as level of temperature stress rises increase in the membrane damage was observed. However the seeds treated with the EBR shows positive effect as there is decrease in the lipid peroxidation in terms of MDA content. Accumulation of proline was also observed in all temperature stress in all three Brassica species. Application of EBR at all concentrations causes significant increase in the proline content as compared with control and untreated.