Effect of agrobacterium induced necrosis, antibiotic induced phytotoxicity and other factors in successful plant transformation

Effect of agrobacterium induced necrosis, antibiotic induced phytotoxicity and other factors in successful plant transformation

Автор: Magdum Sandip S.

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 3 т.9, 2013 года.

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Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L -1 was used for LBA4404 infected Ammi majus calli and Timentin 300 mg L -1 was used for EHA105 infected embryogenic calli of Pearl millet. Effects of Agrobacterium infection, antibiotic and NaOCl washes on Agrobacterium removal and both explants physiological changes during transformation experimental procedures were studied. At the end of the experiments explants survival efficiency of Ammi majus and pearl millet were 8% and 5% respectively. Biotic and abiotic stress factors responsible for lower efficiency were investigated with various other factors and strategies were discussed which are need to be considered for higher transformation events and target tissue survival.

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Abiotic, agrobacterium tumefaciens, ammi majus, biotic, pearl millet, phytotoxicity

Короткий адрес: https://sciup.org/14323791

IDR: 14323791

Список литературы Effect of agrobacterium induced necrosis, antibiotic induced phytotoxicity and other factors in successful plant transformation

  • Alsheikh, M.K., Suso, H.P., Robson, M., Battey, N.H. and Wetten, A. (2002) Appropriate choice of antibiotic and Agrobacterium strain improves transformation of antibiotic-sensitive Fragaria vesca and F. v. semperflorens. Plant Cell Rep., 20: 1173-1180.
  • Assem, S.K., Eissa, H.F. and Saleh, O.M., (2009) Optimization of Agrobacterium-mediated transformation conditions for Egyptian bread wheat CV.G164. Egypt J. Genet. Cytol., 38: 221-234.
  • Barrett, C. and Cassells, A.C. (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium x domesticum cv. 'Grand Slam'. Plant Cell, Tiss. Organ Cult., 36: 169-175.
  • Belide, S., Hac, L., Singh, S.P., Green, A.G. and Wood, C.C. (2011) Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Methods., 7: 1-12.
  • Bown, D. (1995) Encyclopaedia of Herps and their Uses. Dorling Kindersley, London.
  • Ceasar, S.A. and Ignacimuthu, S. (2011) Agrobacterium-mediated transformation of finger millet (Eleusine coracana (L.) Gaertn.) using shoot apex explants. Plant Cell Rep., 30: 1759-1770.
  • Chakrabarty, R., Viswakarma, N., Bhat, S.R., Kirti, P.B., Singh, B.D. and Chopra V.L. (2002) Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower. J. Biosci., 27: 495-502.
  • Cheng, Z.M., Schnurr, J.A. and Kapaun, J.A. (1998) Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Rep., 17: 646-649.
  • Chevallier, A. (1996) The encyclopaedia of medicinal plants. Dorling kindersly, London.
  • Costa, M.G.C., Nogueira, F.T.S., Figueira, M.L., Otoni, W.C., Brommonschenkel, S.H. and Cecon, P.R. (2000) Influence of the antibiotic timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Rep. 19: 327-332.
  • Dan, Y. (2008) Biological functions of antioxidants in plant transformation, In Vitro Cell Dev. Biol. Plant., 44: 149-161.
  • Dan, Y., Munyikawa, T.R.I., Kimberly, A.R. and Rommens, C.M.T. (2004) Use of lipoic acid in plant culture media. US Patent Pub. No.: US 2004/0133938 A1.
  • Das, D., Reddy, M., Upadhyaya, K. and Sopory, S. (2002) An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape (Vitis vinifera L.). Plant Cell Rep. 20: 999-1005.
  • Duan, Y., Zhai, C., Li, H., Li, J., Mei, W., Gui, H., Ni, D., Song, F., Li, L., Zhang, W. and Yang, J. (2012) An efficient and high-throughput protocol for Agrobacterium mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L). Plant Cell Rep., 31: 1611-1624.
  • FAO. (2004) Food and agriculture organization of the United Nations. Pennesitum americanum (L.) Leeke: species description. http://www.fao.org/ag/AGP/AGPC/doc/Gbase/DATA/Pf000297.htm
  • Gonzalez Padilla, I.M., Webb, K. and Scorza, R. (2003) Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants (Prunus domestica L.). Plant Cell Rep., 22: 38-45.
  • Greenberg, J.T., Guo, A., Klessig, D.F. and Ausubel, F.M. (1994) Programmed cell death in plants: a pathogen-triggered response activated coordinately with multiple defense functions. Cell., 77: 551-563.
  • Hamerski, D. and Matern, V. (1988) Elicitor-induced biosynthesis of psoralens in Ammi majus L suspension cultures microsomal conversion of demethylsuberosin into (+) marmesin and psoralen. Eur. J. Biochem., 171: 369-375.
  • Han, S.N., Oh, P.R., Kim, H.S., Heo, H.Y., Moon, J.C., Lee, S.K., Kim, K.H., Seo, Y.W. and Lee, M.O. (2007) Effects of Antibiotics on Suppression of Agrobacterium tumefaciens and Plant Regeneration from Wheat Embryo. J. Crop Sci. Biotech., 10: 92-98.
  • Hansen, G. (2000) Evidence for Agrobacterium-induced apoptosis in maize cells. Mol. Plant Microb. Interact., 13: 649-657.
  • Ieamkhang, S. and Chatchawankanphanich, O. (2005) Augmentin as an alternative antibiotic for growth suppression of Agrobacterium for tomato (Lycopersicon esculentum) transformation. Plant Cell, Tiss. Organ Cult., 82: 213-220.
  • Jha, P., Shashi, Rustagi, A., Agnihotri, P.K., Kulkarni, V.M. and Bhat, V. (2011) Efficient Agrobacterium-mediated transformation of Pennisetum glaucum (L.) R. Br. using shoot apices as explant source. Plant Cell, Tiss. Organ Cult., 107: 501-512.
  • Karthikeyan, A., Pandian, S.K. and Ramesh, P.M. (2011) Agrobacterium-mediated transformation of leaf base derived callus tissues of popular indica rice (Oryza sativa L. sub sp. indica cv. ADT 43). Plant Sci., 181: 258-268.
  • Kneifel, W. and Leonhardt, W. (1992) Testing of different antibiotics against gram positive and gram negative bacteria isolated from plant tissue cultures. Plant Cell, Tiss. Organ Cult., 29: 139-144.
  • Le, V.Q., Belles-Isles, J., Dusabenyagasani, M. and Tremblay, F.M. (2001) An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens. J Exp. Bot., 52: 2089-2095.
  • Leifert, C., Camotta, H. and Waites, W.M. (1992) Effect of combinations of antibiotics on micropropagated Clematis, Delphinium, Hosta, Iris, and Photinia. Plant Cell, Tiss. Organ Cult., 29: 153-160.
  • Leifert, C., Camotta, H., Wright, S.M., Waites, B., Cheyne, V.A. and Waites, W.M. (1991) Elimination of Lactobacillus plantarum, Corynebacterium spp., Staphylococcus saprophyticus and Pseudomonas paucimobilis from micropropagated Hemerocallis, Choisya and Delphinium cultures using antibiotics. J. Appl. Bact., 71: 307-330.
  • Liau, C.H., You, S.J., Prasad, V., Hsiao, H.H., Lu, J.C., Yang, N.S. and Chan, M.T. (2003) Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid. Plant Cell Rep., 21: 993-998.
  • Lin, J.J., Assad-Garcia, N. and Kuo, J. (1995) Plant hormone effect of antibiotics on the transformation efficiency of plant tissue by Agrobacterium tumefaciens cells. Plant Sci., 109: 171-177.
  • Ling, H.Q., Kriseleit, D. and Ganal, M.W. (1998) Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Rep., 17: 843-847.
  • Magdum, S.S. (2013) A Reliable and High Yielding Method for Isolation of Genomic DNA from Ammi majus. I. Res. J. Biological Sci., 2: 57-60.
  • Mamidalaa, P. and Nanna, R.S. (2009) Influence of antibiotics on regeneration efficiency in tomato, Plant Omics Journal, 2: 135-140.
  • Monnier, M. (1990) Induction of Embryogenesis in Suspension Culture. In Pollard, J.W. and Walker, J.M. (ed.), Plant Cell and Tissue Culture. Humana Press, New Jersey, pp. 149-157.
  • Murashige, T. and Skoog, E. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473-497.
  • Nauerby, B., Billing, K. and Wyndaele R. (1997) Influence of the antibiotic timentin on plant regeneration compared to car benicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Sci., 123: 169-177.
  • Okkels, E.T. and Pedersen, M.G. (1988) The toxicity to plant tissue and to Agrobacterium tumefaciens of some antibiotics. Acta Hort., 225: 199-207.
  • Olhoft, P.M., Lin, K., Galbraith, J., Nielsen, N.C. and Somers, D.A. (2001) The role of thiol compounds increasing Agrobacterium-mediated transformation of soybean cotyledonary-node cells. Plant Cell Rep., 20: 731-737.
  • Park, S.U. and Facchini, P.J. (2000) Agrobacterium rhizogenes-mediated transformation of opium poppy, Papaver somniferum l., and California poppy, Eschscholzia californica cham., root cultures. J. Exp. Bot., 51: 1005-1016.
  • Tang, W., Luo, H. and Newton, R.J. (2004) Effects of antibiotics on the elimination of Agrobacterium tumefaciens from loblolly pine (Pinus taeda) zygotic embryo explants and on transgenic plant regeneration. Plant Cell, Tiss. Organ Cult., 70: 71-81.
  • Tereso, S., Miguel, C., Maroco, J. and Oliveira, M.M. (2006) Susceptibility of embryogenic and organogenic tissues of maritime pine (Pinus pinaster) to antibiotics used in Agrobacterium-mediated genetic transformation. Plant Cell, Tiss. Organ Cult., 87: 33-40.
  • Thomzik, J.E. (1995) Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.). In Gartland K.M.A. and Davey, M.R. (ed.), Agrobacterium Protocols. Humana Press Inc., New Jersey, pp. 79-85.
  • Toldi, O., Tóth, S., Pónyi, T. and Scott, P. (2001) An effective and reproducible transformation protocol for the model resurrection plant Craterostigma plantagineum Hochst. Plant Cell Rep., 21: 63-69.
  • Vasil, I.K. (2005) The story of transgenic cereals: The challenge, the debate, and the solution -a historical perspective. In Vitro Cell Dev. Biol., 41: 577-583.
  • Yu, T.A., Yeh, S.D. and Yang, J.S. (2001) Effects of carbenicillin and cefotaxime on callus growth and somatic embryogenesis from adventitious roots of papaya. Bot. Bull. Acad. Sin., 42: 281-286.
  • Zaragozá, C., Muñoz-Bertomeu, J. and Arrillaga, I. (2004) Regeneration of herbicide-tolerant black locust transgenic plants by SAAT. Plant Cell Rep., 22: 832-838.
  • Zheng, Q.S., Ju, B., Liang, L.K. and Xiao, X.H. (2005) Effects of antioxidants on the plant regeneration and GUS expressive frequency of peanut (Arachis hypogaea) explants by Agrobacterium tumefaciens. Plant Cell, Tiss. Organ Cult., 81: 83-89.
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