Effect of Cu and Mn toxicity on chlorophyll fluorescence and gas exchange in rice and sunflower under different light intensities
Автор: Hajiboland R., Hasani B.d
Журнал: Журнал стресс-физиологии и биохимии @jspb
Рубрика: Original article
Статья в выпуске: 1 т.3, 2007 года.
Бесплатный доступ
Copper (Cu) and manganese (Mn) are essential micronutrients for plants, but toxic at high concentrations. Responses of rice (Oryza sativa L.) and sunflower (Helianthus annuus L.) to toxic concentrations of Mn and Cu (up to 100 µM) were studied under three light intensities including low (LL, PPFD=100), intermediate (IL, PPFD=500) and high (HL, PPFD=800) light intensities in hydroponic medium. Rice plants showed higher susceptibility than sunflower to both heavy metals concerning dry matter of shoot and root. Growing under higher light intensity strengthened the effect of Cu toxicity while ameliorated that of Mn, the latter was attributed to the lower Mn accumulation of HL plants in both shoot and root. Chlorophyll content of leaves was influenced negatively only by Cu treatment and that at the highest concentration in the medium (100 µM). Similar with growth results, reduction of net assimilation rate (A) was higher in HL than LL plants treated by excess Cu, but in contrast to growth response, reduction was more prominent in sunflower than rice. Excess Mn-induced reduction of A was similar between LL and HL plants and was greater in sunflower than rice. Reduction of A was partly attributable to stomatal limitation, but non-stomatal mechanisms were also involved in this reduction. Copper and Mn treatment did not change the optimal quantum efficiency of PSII in dark-adapted chloroplasts (Fv/Fm ratio), but Fv/F0 was influenced particularly by Cu treatment, the reduction was higher in rice than sunflower and in HL compared to LL plants. Regarding excess Cu and Mn-mediated alterations in chlorophyll concentration, Fv/F0 and Tm values, it was suggested that, Cu and Mn toxicity depress the leaf photosynthetic capacity primarily by causing a significant alteration of the composition and functional competence of the photosynthetic units rather a reduction in the number of photosynthetic units (PSUs) per unit leaf area.
Cu toxicity, chlorophyll fluorescence, gas exchange, light intensity, mn toxicity
Короткий адрес: https://sciup.org/14323458
IDR: 14323458
Список литературы Effect of Cu and Mn toxicity on chlorophyll fluorescence and gas exchange in rice and sunflower under different light intensities
- Armond, P.A., Bjorkman, O. and Staehlin, L.A. (1980) Dissociation of supra-molecular complexes in chloroplast membranes. A manifestation of heat damage to the photosynthetic apparatus. Biochem. Biophys. Acta, 601, 433-442.
- Asada, K. (1999) The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Ann. Rev. Plant Physiol. PlantMol. Biol., 50, 601-639.
- Behera, R.K. and Choudhury, N.K. (2002) High irradiance induced pigment degradation and loss of photochemical activity of wheat chloroplasts. Biol. Plant, 45 (1), 45-49.
- Dannel, F., Pfeffer, H. and Marschner, H. (1995) Isolation of apoplasmic fluid from sunflower leaves and its use for studies on influence of nitrogen supply on apoplasmic pH. J. Plant Physiol., 146, 273-278.
- De Vos, C.H.R. and Schat, H. (1991) Free radicals and heavy metal tolerance. In Rozeman, J. and Verkleij, J.A. C. (eds.), Ecological Responses to Environmental Stresses Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 22-30.
- Ehleringer, J.R. and Cerling, Т.Е. (1995) Atmospheric CO2 and the ratio of intercellular to ambient CO2 concentrations in plants. Tree Physiol, 15, 105-111.
- Farghuhar, G.D. and Sharkey, T.D. (1982) Stomatal conductance and photosynthesis. Ann. Rev. Plant Physiol., 33, 317-345.
- Fernandes, J.C. and Henriques, F.S. (1991) Biochemical, physiological and structural effects of excess copper in plants. Bot. Rev., 57,246-273.
- Foy, C.D., Chaney, R.L. and White, M.C. (1978) The physiology of metal toxicity in plants. Ann. Rev. Plant Physiol, 29, 511-566.
- Gonzalez, A., Steffen, K.L. and Lynch, P.J. (1998) Light and excess manganese. Implication for oxidative stress in common bean. Plant Physiol., 18, 493-504.
- Gounaries, K., Barber, J. and Harwood, J.L. (1986) The thylakoid membranes of higher plant chloroplasts. Biochem. J., 237, 313-326.
- Hajiboland, R. and Boniadi, H. (2005) Accumulation of copper on root apoplasm and retranslocation to young leaves in rice, maize and sunflower with different toxicity tolerance. Pat J. Biol. Sci., 8 (11), 1599-1609
- Horiguchi, T. (1988) Mechanism of manganese toxicity and tolerance of plants. VII. Effect of light intensity on manganese-induced chlorosis. J. PlantNutr., 11, 235-245.
- Ishida, A., Toma, T. and Matsumoto, Y. (1996) Diurnal changes in leaf gas exchange characteristics in the uppermost canopy of a rain forest tree Dryobalanops aromatica Gaerth. F. Tree Physiol., 16, 779-785.
- Kautsky, H., Appel, W. and Amann, H. (1960) Chlorophyllfluorescenzund kohlensaureassimilation.Biochemishe Zeitschrift, 322, 277-292.
- Kyle, D.J. (1987) The biochemical basis for photoinhibition of photosystem II. In Kyle, D.J., Osmond, C.B. and Arntzen, C.J. (eds.), Photoinhibition, Elsevier, Amsterdam, pp. 197-226.
- Lidon, F.C. (2001) Tolerance of rice to excess manganese in the early stages of vegetative growth. Characterization of manganese accumulation. J. Plant Physiol., 158, 1341-1348.
- Lloyd, J. and Farghuhar, G.D. (1994) 13C determination during CO2 assimilation by the terrestrial biosphere. Oecologia, 99, 201-215.
- Marschner, H. (1995) Mineral Nutrition of Higher Plants. 2nd Edition, Academic Press, UK. Mc Cain, D.C. and Markley, J.L. (1989) More manganese accumulates in maple sun leaves than in shade leaves. Plant Physiol., 90, 1417-1421.
- Maxwell, K. and Johnson, G.N. (2000) Chlorophyll fluorescence-a practical guide. J. Exp. Bot, 51(345), 659-668.
- Moran, R. (1982) Formulae for determination of chlorophyllous pigments extracted with N,N-Dimethylforamide. Plant Physiol., 69, 1376-1381.
- Murkowski, A. (2001) Heat stress and spermidine, effect on chlorophyll fluorescence in tomato plants. Biol. Plant, 44, 53-57.
- Nable, R.O., Houtz, R.L. and Cheniae, G.M. (1988) Early inhibition of photosynthesis during development of Mn toxicity in tobacco. Plant Physiol., 86, 1136-1142.
- Osmond, C.B. (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In Baker, N.R. and Bowyer, J.R. (eds.), Photoinhibition of Photosynthesis, from Molecular Mechanism to the Filed. Bios Scientific Publisher, Oxford, UK, pp. 1-24.
- Ouzounidou, G., Moustakas, M. and Strasser, R. (1997) Sites of action of copper in the photosynthetic apparatus of maize leaves: kinetic analysis of chlorophyll fluorescence oxygen evolution, absorption changes and thermal dissipation as monitored by photoacoustic signals. Aus. J. Plant Physiol, 24, 81-90.
- Ouzounidou, G., Ilias, I., Tranopoulou, H. and Karatagalis, S. (1998) Amelioration of copper toxicity by iron on spinach physiology. J. Plant Nutr., 21,2089-2101.
- Ouzounidou, G., Ilias, I. Kabataidid, M. and Chatzimichail, A. (2003) Comparative study of nutrient deficiencies on growth and photochemistry of tobacco. J. Plant Nutr., 26, 1605-1616.
- Patsikka, E., Aro, E-M. and Tyystjarvi, E. (1998) Increase in the quantum yield of photoinhibition contributes to copper toxicity in vivo. Plant Physiol, 117, 619-627.
- Patsikka, E., Aro, E-M. and Tyystjarvi, E. (2001) Mechanismofcopper-enhanced photoinhibition in thylakoid membranes. Physiol. Plant, 113, 142-150.
- Patsikka E., Kairavuo, M., Sersen, F., Aro, E-M. and Tyystjarvi, E. (2002) Excess copper predisposes photosystem II to photoinhibition in vivo by outcompeting iron and causing decrease in leaf chlorophyll. Plant Physiol., 129, 1359-1367.
- Pereira, W.E., de Siqueira, D.L., Martynez, C.A. and Puiatti, M. (2000) Gas exchange and chlorophyll fluorescence in four citrus rootstocks under aluminium stress. J. Plant Physiol., 157,513-520.
- Perfus-Barbeoch, L., Leonhardt, N., Vavasseur, A. and Forestier, С (2002) Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the water status. The Plant J., 32 (4), 539-548.
- Quartacci, M.F., Pinzino, C, Sgherri, C.L.M., Dalla Vecchia, F., and Navari-Izzo, F. (2000) Growth in excess copper induces changes in the lipid composition and fluidity of PSII-enriched membranes in wheat. Physiol. Plant, 108, 87-93.
- Souza, R.P., Machadoa, E.C. Silva, J.A.B., Lagoa, A.M.M.A. and Silveria, J.A.G. (2004) Photosynthetic gas exchange, chlorophyll fluorescence and some associated metabolic changes in cowpea (Vigna unguiculata) during water stress and recovery. Environ. Exp. Bot, 51, 45-56.
- Stiborova, M., Doubravova, M. Brezinova, A. and Friedrich, A. (1986) Effect of heavy metal ions on growth and biochemical characteristics of maize (Zea mays L.). Biologia, 20, 418-425.
- Tennant, D. (1975) A test of modified line intersect method of estimating root length. J. Ecology, 63, 995-1001.
- Vichnevetskaia, K.D. and Roy, D.N. (1999) Oxidative stress and antioxidative defense with an emphasis on plants antioxidants. Environ. Rev., 7,31-51.
- Weckx, J.E.J., and Clijsters, H.M.M. (1997) Zinc phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris. Plant Physiol. Biochem., 35, 405-410.
- Wissemeier, A.H. and Horst, W.J. (1992) Effect of light intensity on manganese toxicity symptoms and callose formation in cowpea (Vigna unguiculata L. Walp.). Plant Sci., 143, 299-309.
- Woolhouse, H.W. (1983) Toxicity and tolerance in the responses of plants to metals. In Lange O.L., Nobel, P.S., Osmond, C.B. and Ziegler, H. (Eds.). Physiological Plant Ecology III: Responses to the chemical and biological environment. Springer, Berlin, Germany, pp. 245-300.
- Xu, Q., Paulsen, A.Q. Guikema, J.A. and Paulsen, G.M. (1995) Functional and ultrastractural injury to photosynthesis in wheat by high temperature during maturation. Env. Exp. Bot, 35, 43-54.
- Yang, H.M., Zhang, X.Y. and Wang, G.X. (2004) Effects of heavy metals on stomatal movements in broad bean leaves. Russ. J. Plant Physiol., 51 (4), 464-468.
- Ymela, I., Gatzen, G., Picorel, R., Holzwarth, A.R. (1996) Cu (Il)-inhibitory effect on photosystem II from higher plants: a picosecond time-resolved fluorescence study. Biochemistry, 35, 9469-9474.
- Yoshida, S., Forno, D.A., Cock, J. H. and Gomez, K. (1972) Routine methods of solution culture for rice. In Laboratory Manual for Physiological Studies of Rice. 2nd Edition, The International Rice Research Institute, Philippines, pp. 53-57
