Efficient somatic embryogenesis and plantlet regeneration induced from mature embryo culture under heavy metals stress conditions in a millet crop sorghum [Sorghum bicolor (L.) Moench]

Efficient somatic embryogenesis and plantlet regeneration induced from mature embryo culture under heavy metals stress conditions in a millet crop sorghum [Sorghum bicolor (L.) Moench]

Автор: Roselin Roobavathi M., Vikrant

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 1 т.21, 2025 года.

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The present study aims to establish the somatic embryogenesis and plantlet regeneration in Sorghum bicolor ( cv. PAC501) under heavy metals (lead and cadmium) stress conditions. Mature embryos were inoculated in MS-medium containing (10mg/L, 25mg/L, 50mg/L, 75mg/L, and 100mg/L) of PbSO4 and CdCl2 (10mg/L, 25mg/L, 50mg/L, 75mg/L, 100mg/L, 150mg/L, and 200mg/L) each with IAA (1.0mg/L), BAP (0.5mg/L), zeatin (0.1mg/L), and proline (0.7mg/L). Significantly, high frequency (87.2±0.21%) of somatic embryogenesis was initially obtained with IAA (1.0mg/L), BAP (0.5mg/L), zeatin (0.1mg/L), and proline (0.7mg/L) while in comparison, 2,4-D (2.5mg/L) along with same concentrations of BAP, zeatin, and proline was proved as relatively less efficient (67.4±0.64%) for somatic embryogenesis. Furthermore, results reveal that sub-culture of embryogenic callus on cytokinins; BAP, Kn, and Zn (0.1mg/L, 0.5mg/L, and 1.0mg/L) of each added regeneration medium, kinetin (0.5mg/L) was recorded as the most effective cytokinin (77.8±0.93%) for plantlets regeneration. Moreover, during heavy metals treatments, the least frequency (2.9±3.32%) of lead tolerant somatic embryos was obtained with PbSO4 (75mg/L) in presence of the same concentrations of IAA, BAP, zeatin, and proline. Significantly, the very low frequency (3.7±0.67%) of lead tolerant plantlets regeneration was recorded with PbSO4 (75mg/L) and Kn (0.5mg/L). In contrast, during CdCl2 stress treatment, the cadmium tolerant somatic embryos (3.1±3.24%) were obtained with CdCl2 (150mg/L) and IAA, BAP, zeatin, and proline. Further, cadmium tolerant plantlets (4.23±2.33%) were also achieved on medium containing kinetin (0.5mg/L) with CdCl2 (100mg/L) indicating that lead proves to be more toxic for somatic embryogenesis and plantlets regeneration than cadmium. Later, the regenerated tolerant plantlets were transferred to pots and gradually acclimatized in greenhouse.

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Heavy metal, millets, mature embryo, regeneration, somatic embryogenesis, sorghum

Короткий адрес: https://sciup.org/143183772

IDR: 143183772

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