EPCAM+CD45+ cells in ascitic fluid of patients with ovarian cancer: a relationship with tumor marker levels and tumor grade

Purpose of the study: to assess the relationship between atypical/hybrid forms of EpCAM+CD45+ cells in ascitic fluid of ovarian cancer patients and the levels of cancer markers, such as CA125 and HE4, and the tumor grade. Material and Methods. The study included 48 patients with newly diagnosed ovarian cancer (42 patients with stage Ic-IV ovarian cancer and 6 patients with borderline ovarian tumors (BOTs). The age of the patients ranged from 36 to 76 years. The study material included ascitic fluid and blood samples. The presence of atypical/hybrid forms of EpCAM+CD45+ cells in ascitic fluid was identified by laser multicolor flow cytometry. The levels of CA125 and HE4 markers were measured by ELISA. Results. The number of EpCAM+CD45+ cells in ascitic fluid of patients with serous ovarian carcinoma was 1.02 (0.30; 2.68) cells/pl (0.55 (0.03; 4.51) cells/pl in patients with low-grade serous carcinoma (LGSC) and 1.36 (0.41; 2.68) cells/pl in patients with high-grade serous carcinoma (HGSC). The number of EpCAM+CD45+ cells in ascitic fluid of serous ovarian carcinoma was shown to have a strong correlation with СА125 and HE4 levels in blood serum (R=0.60; р function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }