Evaluation of some upland cotton ( Gossypium hirsutum L.) varieties for salinity tolerance screening
Автор: Saleh Basel
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 3 т.9, 2013 года.
Бесплатный доступ
This investigation aimed to evaluate five upland cotton ( Gossypium hirsutum L.) (Aleppo118, Aleppo33/1, Aleppo90, Raqqa5, and Deir-Ezzor22) varieties based on physiological indices and genetic variation using AFLP marker. These varieties were evaluated under control and saline conditions (50, 100 & 200 mM NaCl) for 56 days. Various physiological indices were detected in this investigation. In this respect, Deir-Ezzor22 variety differed by showing high salt tolerance relative to the other tested varieties. Otherwise, PCR amplification with 7 AFLP PCs primer combinations revealed that Deir-Ezzor22 variety characterized by 15 unique positive markers compared to the other tested varieties. Based on physiological study and AFLP technique, it can be concluded that genetic variation detected by AFLP marker supported the physiological indices among the tested cotton varieties. These varieties present considerable interest for genetic studies and plant improvement.
Aflp marker, cotton, physiological parameters, salt stress
Короткий адрес: https://sciup.org/14323785
IDR: 14323785
Список литературы Evaluation of some upland cotton ( Gossypium hirsutum L.) varieties for salinity tolerance screening
- Abacus Concept (1996) Statview 4.5 Statistical Program Abacus Concepts Corporation, Berkeley, CA, USA.
- Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant. Physiol., 24: 1-15.
- Atienzar, F.A., Conradi, M., Evenden, A.J., Jha, A.N. and Depledge, M.H. (1999) Qualitative assessment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna expose to benzo [a] pyrene. Environ. Toxicol. & Chem., 18: 2275-2282.
- Badigannavar, A., Myers, G., Agcenter, L.S.U. and Baton-Rouge, L.A. (2010) Genetic analysis of AFLP markers associated with seed quality traits in Upland cotton (Gossypium hirsutum). 2010 Beltwide Cotton Conferences, New Orleans, Louisiana, January 4-7. Nat. Cotton Counc. Am., Memphis, TN.
- Chaudhary, L., Sindhu, A., Kumar, R. and Saini, M. (2010) Estimation of genetic divergence among some cotton varieties by RAPD analysis. J. Plant. Breed. Crop. Sci., 2(3): 39-43.
- Doyle, J.J. and Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytoch. Bull., 19: 11-15.
- El-Zaeem, S.Y. (2012) Identification of different selected genotypes of salinity resistance of each of full-sib Nile tilapia, Blue tilapia and their diallel interspecific hybridization using random amplified polymorphic DNA fingerprinting. Afr. J. Biotech., 11(35): 8645-8652.
- Gupta, M. and Sarin, N.B. (2009) Heavy metal induced DNA changes in aquatic macrophytes: Random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker. J. Environ. Sci., 21: 686-690.
- Hoagland, D.R. and Arnon, D.I. (1950). The water culture method for growing plants without soil. Circular 347, College of Agriculture, University of California, Berkeley; College Agriculture circular No. 347.
- Hussein, E.H.A., Marwa, H.A., Hussein, O.M.H. and Adawy, S.S. (2007) Molecular characterization of cotton genotypes using PCR-based markers. J. Appl. Sci. Res., 3(10): 1156-1169.
- Kurup, S.S., Hedar, Y.S., Al Dhaheri, M.A., El-Heawiety, A.Y., Aly, M.A.M. and Alhadrami, G. (2009) Morpho-physiological evaluation and RAPD markers-assisted characterization of date palm (Phoenix dactylifera L.) varieties for salinity tolerance. J. Food. Agric. & Environ., 7 (3&4): 303-507.
- Moradi, A., Tahmourespour, A., Hoodaji, M. and Khorsandi, F. (2011). Effect of salinity on free living -diazotroph and total bacterial populations of two saline soils. Afr. J. Microb. Res., 5 (2): 144-148.
- Saleh, B. (2011a) Effect of salt stress (NaCl) on biomass and K+/Na+ ratio in cotton. J. Stress. Physiol. & Biochem., 7 (4): 05-14.
- Saleh, B. (2011b) Ion partitioning and Mg2+/Na+ ratio under salt stress application in cotton. J. Stress. Physiol. & Biochem., 7 (4): 292-300.
- Saleh, B. (2012) Effect of salt stress on growth and chlorophyll content of some cultivated cotton varieties grown in Syria. Comm. Soil. Sci. Plant. Anal., 43 (15): 1976 -1983.
- Savva, D. (1998) Use of DNA fingerprinting to detect genotoxic effects. Ecotoxic. Environ. Saf., 41:103-106.
- Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kulper, M. and Jabeau, M. (1995) AFLP: a new technique for DNA fingerprinting. Nucl. Acid Res., 23: 4407-4414.
- Zhou, L., Li, J., Lin, X. and Al-Rasheid, K.A.S. (2011) Use of RAPD to detect DNA damage induced by nitrofurazone in marine ciliate Euplotes vannus (Protozoa, Ciliophora). Aquat. Toxicol., 103: 225-232.