Evaluation of the cytotoxic effects and apoptosis inducing of Cuscuta epithymum extract on esophageal squamous cell carcinoma

Evaluation of the cytotoxic effects and apoptosis inducing of Cuscuta epithymum extract on esophageal squamous cell carcinoma

Автор: Hashemi M., Panahi A., Nosrati R., Oranj G.D., Jafari-shakib R.

Журнал: Сибирский онкологический журнал @siboncoj

Рубрика: Лабораторные и экспериментальные исследования

Статья в выпуске: 4 т.23, 2024 года.

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Background. Esophageal cancer is the eighth most common cancer in the world. The antitumor effects of medicinal plants have been shown as a therapeutic strategy to treat esophageal cancer. This study aimed to evaluate in vitro effects of Cuscuta epithymum extract on the survival and apoptosis of esophageal cancer cell line. Material and Methods. Here, the hydroalcoholic Soxhlet extract of C. epithymum plant was prepared. The cell viability of esophageal cancer cell line KYSE-30 was evaluated by MTT assay after 24 h treatment with concentrations of 50, 100, 200, 400, 600, 800 and 1000 μg/ml of the extract. Then, the apoptotic effect of extract was evaluated by flow cytometry using Propidium Iodide (PI) staining and sub-G1 peak analysis, and Annexin V-FITC/PI staining in cells treated with concentrations of 125, 250, 500 and 750 μg/ml as well as morphological change of healthy cells to apoptotic and necrotic form.

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Cuscuta extract, cuscuta epithymum, mtt, esophageal cancer, apoptosis

Короткий адрес: https://sciup.org/140307092

IDR: 140307092   |   DOI: 10.21294/1814-4861-2024-23-4-77-85

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