Evaluation of the effect of oxidative stress on roots of red beet ( Beta vulgaris L.)

Автор: Kolesnikova E.V., Ozolina N.V., Nurminsky V.N., Nesterkina I.S., Sitneva L.A., Lapteva T.I.

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 4 т.10, 2014 года.

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In the present investigation it was studied the effect of oxidative stress on root of red beet. The degree of stress exposure was assessed at various levels: in tissues of roots (conductometric method), on isolated vacuoles (time-lapse video recording), and transport activity of the vacuolar membrane enzyme, namely V-H +-ATPase (fluorescent probe method). The obtained results allow to conclude the negative effects of oxidative stress on different levels of plant organization. There were several effects: a significant increase of outflow of electrolytes from the tissues of roots, a great decrease of half-life time of isolated vacuoles, and a 2 times reduction of transport activity of tonoplast H +-ATPase. Thus, the methods used in this study can be applied to assess the intensity of the impact of oxidative stress on plants.

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Enzyme activity, electrolyte outflow, root of red beet, oxidative stress, stability of vacuoles

Короткий адрес: https://sciup.org/14323909

IDR: 14323909

Список литературы Evaluation of the effect of oxidative stress on roots of red beet ( Beta vulgaris L.)

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