Expression of miRNAs confers enhanced tolerance to drought and salt stress in finger millet ( Eleusine coracona)
Автор: Nageshbabu R., Usha , Jyothi M.N., Sharadamma N., Rai D.V., Devaraj V.R.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 3 т.9, 2013 года.
Бесплатный доступ
Plants respond to the environmental cues in various ways, recent knowledge of RNA interference in conferring stress tolerance had become a new hope of developing tolerant varieties. Here we attempt to unfold the molecular mechanism of stress tolerance through miRNA profiling and expression analysis in Finger millet (Eleusine coracona) under salt and drought stress conditions. The expression analysis of 12 stress specific conserved miRNAs was studied using semi-quantitative real time PCR and Northern blot assay. Our studies revealed that, although most of the miRNAs responded to the stresses, the expression of particular miRNA differed with the nature of stress and the tissue. The expression analysis was correlated with the existing data of their target genes. Abiotic stress up-regulated miRNAs are expected to target negative regulators of stress responses or positive regulators of processes that are inhibited by stresses. On the other hand, stress down-regulated miRNAs may repress the expression of positive regulators and/or stress up-regulated genes. Thus the current study of miRNAs and their targets under abiotic stress conditions displays miRNAs may be good candidates to attribute the stress tolerance in plants by transgenic technology.
Drought, finger millet, mirna, salt stress
Короткий адрес: https://sciup.org/14323767
IDR: 14323767
Список литературы Expression of miRNAs confers enhanced tolerance to drought and salt stress in finger millet ( Eleusine coracona)
- Aukerman M.J, Sakai H. (2003) Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell 15: 2730-2741.
- Chuck G, Cigan A.M, Saeteurn K, Hake S. (2007) The heterochronic maize mutant Corngrass1 results from over expression of tandem microRNA. Nat Genet 39: 544-549.
- Ding D, Zhang L, Wang H, Liu Z, Zhang Z. (2009) Differential expression of miRNAs in response to salt stress in maize roots. Ann Bot 103: 29-38.
- Ding Y, Chen Z, and Zhu C. (2011) Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa). J Exp Bot 62: 3563-3573.
- Eckardt A. (2012) A MicroRNA Cascade in Plant Defense. Plant cell 24: 840.
- Gou J.Y, Felippes F.F, Liu C.J, Weigel D, Wang J.W. (2011) Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor. Plant Cell 23: 1512-1522.
- Hui Li, Ying Deng, Tianlong Wu, Senthil Subramanian, Oliver Yu. (2010) Mis-expression of miR482, miR1512, and miR1515 increases soybean nodulation. Plant Physiology 153(4) 1759-1770.
- Kwon H.B, Hwang E.W, Shin S.J, Park S.C, Jeong M.J. (2011) Identification of miR172 family members and their putative targets responding to drought stress in Solanum tuberosum. Genes & Genomics 33: 105-110.
- Li B, Qin Y, Duan H, Yin W, Xia X. (2011) Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica. J. Exp. Bot. 62(11): 3765-3779.
- Liu H.H, Tian X, Li Y.J, Wu C.A, Zheng C.C. (2008). Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. RNA-a Publication of the RNA Society 14: 836-843.
- Matts J, Jagadeeswaran G, Roe B.A, Sunkar R. (2010) Identification of microRNAs and their targets in switch grass, a model biofuel plant species. Journal of Plant Physiology 167: 896-904.
- Park C.M, Jung J.H, Seo P.J, Kang S.K. (2011) miR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitions. Plant Molecular Biology 76: 35-45.
- Poethig R.S, Wu G, Park M.Y, Conway S.R, Wang J.W. (2009) The Sequential Action of miR156 and miR172 Regulates Developmental Timing in Arabidopsis. Cell. 138: 750-759.
- Sunkar R. (2010) MicroRNAs with macro-effects on plant stress responses. Seminars in Cell & Developmental Biology 21: 805-811.
- Tianzuo Wang, Lei Chen, Mingui Zhao, Qiuying Tian and Wen-Hao Zhang. (2011) Identification of drought responsive microRNAs in Medicago truncatula by genome-wide high-throughput Sequencing. BMC Genomics 12: 367-378.
- Wang J.W, Wang L.J, Mao Y.B, Cai W.J, Xue H.W, Chen X.Y. (2005) Control of root cap formation by MicroRNA-targeted auxin response factors in Arabidopsis. Plant Cell, 17(8): 2204-2216.
- Wang J.W, Czech B, Weigel D. (2009) miR156 regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. Cell, 138: 738-749.
- Xie F, Frazier T.P, Zhang B. (2010). Identification and characterization of microRNAs and their targets in the bio-energy plant switch grass (Panicum virgatum). Planta 232: 417-434.
- Xing S, Salinas M, Hohmann S, Berndtgen R, Huijser P. (2010) miR156 targeted and non-targeted SBP-box transcription factors act in concert to secure male fertility in Arabidopsis. Plant Cell, 22: 3935-3950.
- Zhang B, Pan X. (2009). Expression of microRNAs in cotton. Mol Biotechnol, 42: 269-274.
- Zhao B, Ge L, Liang R, Li W, Ruan K, (2009) Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor. BMC Mol Biol 10: 29-38.
- Zhou L, Liu Y, Liu Z, Kong D, Duan M. (2010) Genome-wide identification and analysis of drought-responsive microRNAs in Oryza sativa. J Exp Bot 61: 4157-4168.
- Zhu J.K, Li W.X, Oono Y, Zhu J.H, He X.J. (2008) The Arabidopsis NFYA5 transcription factor is regulated transcriptionaly and post-transcriptionaly to promote drought resistance. Plant Cell 20: 2238-2251.
