Features of Blood Crystallostasis Modulation by the Dinitrosyl Iron Complex: In vitro Study

The aim of the work was to evaluate the effect of free and deposited in the composition of dinitrosyl iron (DNIC) complexes of nitrogen monoxide on the crystallogenic properties and metabolic parameters of blood serum in vitro. Material and methods. The effect of various forms of nitric oxide (NO-containing gas mixtures from the Plason apparatus (initial or tenfold diluted gas flow – 80 and 800 ppm) and an experimental nitric oxide generator (20-100 ppm), glutathione-containing DNIC) on the crystallization of blood serum was studied in vitro. Shifts in the crystallogenic properties of biofluid were compared with changes in other physical and chemical parameters. Results. It has been shown that different forms of nitric oxide have different effects on the crystallogenic properties of blood serum in vitro, and the feature of the action of DNIC is the most pronounced stimulation of the structuring of biofluid (an increase in crystallizability by 1.28 times, and the structure index by 1.47 times), combined with a decrease in the destruction of elements (by 1.95 times) and an expansion of the marginal zone (in 1.56 times). It was revealed that these shifts correlate with the dynamics of other metabolic and physico-chemical parameters.