Features of microcirculatory responses in experimental wound area in white rats

Автор: Darya D. Loiko, Angelina A. Savkina, Tatyana V. Stepanova, Tatyana S. Kiriyazi, Oleg V. Osnovin, Tamara A. Andronova, Ilmira I. Abdrakhmanova, Artyom N. Fedorov, Aleksey N. Ivanov

Журнал: Saratov Medical Journal @sarmj

Статья в выпуске: 3 Vol.3, 2022 года.

Бесплатный доступ

Objective: to examine changes in the microcirculatory bed parameters via laser Doppler flowmetry in the course of wound healing and the possibility of their use for upgrading the technology of evaluating the effectiveness of wound healing agents. Materials and Methods. The studies were performed on 25 white rats distributed between two groups: 10 control animals (intact rats) and 15 animals with a full-thickness experimental skin defect. The microcirculation parameters in the skin of experimental wound edges in rats were evaluated using laser Doppler flowmetry, and histological preparations of tissues in the wound area were analyzed. Results. Changes in skin microcirculation at the wound edges were characterized by inflammatory hyperemia manifested by an increase in the perfusion index by 27% and augmented normalized amplitudes of myogenic, respiratory and cardiogenic oscillations. Changes in microcirculation were verified by the morphological picture of inflammation, which reflects an increase in the number of vessels fully filled with arterial and venous blood, as well as in leukocyte infiltration of the wound edges and bottom. Conclusion. Monitoring of microcirculatory disorders occurring in the area of skin wounds allows assessing the dynamics of the reparative process, which could be used for developing and evaluating the effectiveness of existing medicamentous and non-medicamentous methods of stimulating regeneration.

Еще

Microcirculation, regeneration, full-thickness skin wound

Короткий адрес: https://sciup.org/149146156

IDR: 149146156   |   DOI: 10.15275/sarmj.2022.0303

Список литературы Features of microcirculatory responses in experimental wound area in white rats

  • Sen CK. Human wound and its burden: Updated 2020 compendium of estimates. Adv Wound Care (New Rochelle) 2021; 10 (5): 281–92. https://doi.org/10.1089/wound.2021.0026
  • Haller HL, Sander F, Popp D, et al. Oxygen, pH, lactate, and metabolism—How old knowledge and new insights might be combined for new wound treatment. Medicina (Kaunas) 2021; 57 (11): 1190. https://doi.org/10.3390/medicina57111190
  • Yousefi S, Qin J, Dziennis S, et al. Assessment of microcirculation dynamics during cutaneous wound healing phases in vivo using optical microangiography. J Biomed Opt 2014; 19 (7): 76015. https://doi.org/10.1117/1.JBO.19.7.076015
  • Sоrensen MA, Petersen LJ, Bundgaard L, et al. Regional disturbances in blood flow and metabolism in equine limb wound healing with formation of exuberant granulation tissue. Wound Repair Regen 2014; 22 (5): 647–53. https://doi.org/10.1111/wrr.12207
  • Kulikov DA, Glazkov AA, Kovaleva YuA. et al. Prospects for the use of laser Doppler flowmetry application in assessment of skin microcirculation in diabetes mellitus. Diabetes Mellitus 2017; 20 (4): 279–85. [In Russ.] https://doi.org/10.14341/DM8014
  • Smotrin SM, Dovnar RI, Vasilkov AYu, et al. Effect of a dressing containing gold or silver nanoparticles on experimental wound healing. Journal of Grodno State Medical University 2012; 1 (37): 75–80. [In Russ.]
  • Humeau A, Koïtka A, Abraham P, et al. Time-frequency analysis of laser Doppler flowmetry signals recorded in response to a progressive pressure applied locally on anaesthetized healthy rats. Phys Med Biol 2004; 49 (5): 843–57. https://doi.org/10.1088/0031-9155/49/5/014
  • Bi H, Feng T, Li B, et al. In vitro and in vivo comparison study of electrospun pla and pla/pva/sa fiber membranes for wound healing. Polymers (Basel) 2020; 12 (4): 839. https://doi.org/10.3390/polym12040839
  • Krupatkin AI. Blood flow oscillations as a new diagnostic language in the study of microcirculation. Regional Blood Circulation and Microcirculation 2014; 13 (1): 83–99. Russian https://doi.org/10.24884/1682-6655-2014-13-1-83-99
  • Tecchio C, Cassatella MA. Neutrophil-derived cytokines involved in physiological and pathological angiogenesis. Chem Immunol Allergy 2014; (99): 123–37. https://doi.org/10.1159/000353358
  • Wang Z, Qi F, Luo H, Xu G, et al. Inflammatory microenvironment of skin wounds. Front Immunol 2022; (13): 789274. https://doi.org/10.3389/fimmu.2022.789274
Еще
Статья научная